Longhurst P A, Schwegel T, Folander K, Swanson R
Division of Urology, Hospital of the University of Pennsylvania, Philadelphia, USA.
Biochim Biophys Acta. 1996 Sep 11;1308(3):185-8. doi: 10.1016/0167-4781(96)00112-1.
A human urinary bladder cDNA library was screened with a rat P2x purinoceptor probe. A full-length cDNA was isolated, and like its rat homologue, the deduced protein consists of 399 amino acids (M(r) = 44980 Da), contains two hydrophobic, putative transmembrane, domains flanking a large presumed extracellular loop, and represents the P2x1 subtype of this multigene family (approximately 89% amino acid sequence identity to rat P2x1, but only 40-50% identity to rat P2x2-4). Expression of the P2x1 gene in human bladder was confirmed by Northern analysis, which demonstrated a major transcript of approximately 2.9 kb. Transcripts were also found in a variety of other tissues including adult peripheral leukocytes, pancreas, spleen, prostate, small intestine, colon, testis, and ovary, and in fetal liver. The gene encoding the human P2x1 receptor was localized to chromosome 17 by Southern analysis of DNAs isolated from a panel of somatic cell hybrids. The results support a role for P2x purinoceptors in the regulation of human bladder function.
用人膀胱cDNA文库以大鼠P2x嘌呤受体探针进行筛选。分离出一个全长cDNA，与其大鼠同源物一样，推导的蛋白质由399个氨基酸组成（分子量=44980道尔顿），包含两个疏水的、推测的跨膜结构域，两侧为一个大的推测的细胞外环，代表该多基因家族的P2x1亚型（与大鼠P2x1的氨基酸序列同一性约为89%，但与大鼠P2x2 - 4的同一性仅为40 - 50%）。通过Northern分析证实了P2x1基因在人膀胱中的表达，该分析显示了一个约2.9kb的主要转录本。在包括成人外周血白细胞、胰腺、脾脏、前列腺、小肠、结肠、睾丸和卵巢以及胎儿肝脏在内的多种其他组织中也发现了转录本。通过对从一组体细胞杂种中分离的DNA进行Southern分析，将编码人P2x1受体的基因定位到染色体17上。这些结果支持P2x嘌呤受体在调节人膀胱功能中起作用。
