干扰素-γ（IFN-γ）可以抵消HIV p24免疫抑制七肽在体外的抑制作用。
Interferon-gamma (IFN-gamma) can counteract the in vitro inhibitory effect of an HIV p24 immunosuppressive heptapeptide.
作者信息
Luzzati A L, Boirivant M, Giacomini E, Giordani L, Di Modugno F, Chersi A
机构信息
Department of Immunology, Istituto Superiore di Sanità, Rome, Italy.
出版信息
Clin Exp Immunol. 1996 Sep;105(3):403-8. doi: 10.1046/j.1365-2249.1996.d01-778.x.
Previous work from our laboratory demonstrated that a synthetic heptapeptide (Ch7), corresponding to a conserved sequence of HIV core protein p24 (aa 232-238), was able to specifically abrogate antigen-induced responses in cultures of normal human peripheral blood lymphocytes (PBL). In the present study we show that Ch7 did not inhibit the induction of IFN-gamma-secreting cells nor the accumulation of IFN-gamma mRNA in antigen-stimulated cultures. However, delayed addition of recombinant human IFN-gamma to Ch7-suppressed cultures was able to restore fully the capacity to mount an antigen-specific antibody response. Thus, although the Ch7 immunosuppressive effect may not be directly related to a decreased production of IFN-gamma, an increased level of this cytokine is certainly able to counteract the negative effect of the peptide.
我们实验室之前的研究表明，一种合成七肽（Ch7），对应于HIV核心蛋白p24的保守序列（氨基酸232 - 238），能够特异性消除正常人外周血淋巴细胞（PBL）培养物中抗原诱导的反应。在本研究中，我们发现Ch7并不抑制抗原刺激培养物中分泌γ干扰素的细胞的诱导，也不抑制γ干扰素mRNA的积累。然而，在Ch7抑制的培养物中延迟添加重组人γ干扰素能够完全恢复产生抗原特异性抗体反应的能力。因此，尽管Ch7的免疫抑制作用可能与γ干扰素产生减少没有直接关系，但这种细胞因子水平的升高肯定能够抵消该肽的负面影响。
Zotero 插件