Müller-Holzner E, Marth C, Zeimet A G, Hofstädter F, Daxenbichler G
Department of Obstetrics and Gynecology, University Hospital, Innsbruck, Austria.
J Steroid Biochem Mol Biol. 1996 Apr;58(1):13-9. doi: 10.1016/0960-0760(96)81485-3.
The effects of estradiol, medroxyprogesterone acetate (MPA) dexamethasone, dihydrotestosterone and the antihormones 4-OH tamoxifen and RU 38486 were studied in two established breast carcinoma cell lines, the estrogen-sensitive ZR-75-1 and the estrogen-independent BT 20 cells applying two different in vitro systems, spheroid and monolayer cell culture in steroid deprived medium. Growth of ZR-75-1 spheroids was dramatically stimulated by the addition of estradiol, an effect which was neutralized by the simultaneous addition of 4-OH tamoxifen. The antiestrogen alone as well as dihydrotestosterone and MPA reduced ZR-75-1 spheroid growth significantly. While growth of BT 20 spheroids was only transiently inhibited by tamoxifen and dihydrotestosterone, a persistent increase in BT 20 spheroid growth was observed under MPA treatment in a concentration of 1 microM. This effect, although statistically significant, was very moderate. With the exception of this finding, growth effects of the different test compounds were similar in both in vitro systems, tumor spheroids and monolayer cell cultures.
在两种已建立的乳腺癌细胞系中，即雌激素敏感的ZR - 75 - 1细胞系和雌激素非依赖的BT 20细胞系，运用两种不同的体外系统，即无类固醇培养基中的球体培养和单层细胞培养，研究了雌二醇、醋酸甲羟孕酮（MPA）、地塞米松、双氢睾酮以及抗激素4 - 羟基他莫昔芬和RU 38486的作用。添加雌二醇可显著刺激ZR - 75 - 1球体的生长，而同时添加4 - 羟基他莫昔芬可中和这一效应。单独使用抗雌激素以及双氢睾酮和MPA均可显著降低ZR - 75 - 1球体的生长。虽然他莫昔芬和双氢睾酮仅短暂抑制BT 20球体的生长，但在1微摩尔浓度的MPA处理下，观察到BT 20球体生长持续增加。尽管这一效应具有统计学意义，但非常微弱。除了这一发现外，不同测试化合物在两种体外系统，即肿瘤球体和单层细胞培养中的生长效应相似。
