Gage J P, Shaw R M, Moloney F B
Department of Dentistry, University of Queensland, Brisbane, Australia.
J Prosthet Dent. 1995 Nov;74(5):517-20. doi: 10.1016/s0022-3913(05)80355-5.
Controlled studies were undertaken to ascertain the nature of the fibrous tissue component of surgically removed dysfunctional human temporomandibular joint disks. Each disk was divided into several parts and dissociative methods were used to extract collagenous residues. The resultant residues were digested with cyanogen bromide and the obtained soluble peptides were characterized by their electrophoretic mobility on polyacrylamide slab gels. The separated peptides were then scanned on a laser densitometer. Type III collagen was identified in samples taken from both the women's and the men's posterior disk attachment tissues. The amount of type III collagen in the women's attachments was approximately twice that found in the men's and both of these quantities were greater than those previously found in nondysfunctional tissues. The finding that there was more type III collagen in the posterior attachments of the women's chronically damaged disks may indicate that these tissues in women are less able to withstand functional loading.
开展了对照研究，以确定手术切除的功能失调的人类颞下颌关节盘纤维组织成分的性质。将每个关节盘分成几个部分，并采用解离方法提取胶原残留物。所得残留物用溴化氰消化，通过在聚丙烯酰胺平板凝胶上的电泳迁移率对获得的可溶性肽进行表征。然后用激光密度计对分离出的肽进行扫描。在取自女性和男性后盘附着组织的样本中均鉴定出III型胶原。女性附着组织中III型胶原的量约为男性的两倍，且这两个量均大于先前在无功能障碍组织中发现的量。女性慢性受损关节盘后附着组织中III型胶原较多这一发现可能表明，女性的这些组织承受功能负荷的能力较弱。
