狗肝脏3-羟基-3-甲基戊二酰辅酶A还原酶。
Dog liver 3-hydroxy-3-methylglutaryl coenzyme A reductase.
作者信息
Ho P P, Esterman M A, Towner R D, Turner R K
出版信息
Enzyme. 1977;22(4):242-8.
A rapid and sensitive assay method for 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-coA reductase, EC 1.1.1.34) is described. HMG-coA reductase is demonstrated in dog liver microsomes, and the converted reaction product has been identified as mevalonolactone. The enzyme activity undergoes cyclic variation and increases by more than tenfold 5 h after feeding. The properties of dog liver enzyme are generally similar to the rat liver enzyme in the method of solubilization, cold inactivation, pH optimum, and Km values.
本文描述了一种用于检测3-羟基-3-甲基戊二酰辅酶A还原酶（HMG-CoA还原酶，EC 1.1.1.34）的快速灵敏的检测方法。在犬肝微粒体中证实了HMG-CoA还原酶的存在，并且已鉴定出转化后的反应产物为甲羟戊酸内酯。该酶活性呈周期性变化，喂食后5小时增加超过十倍。犬肝酶的性质在溶解方法、冷失活、最适pH值和米氏常数方面通常与大鼠肝酶相似。
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