Norton P M, Brown H W, Wells J M, Macpherson A M, Wilson P W, Le Page R W
Department of Pathology, University of Cambridge, UK.
FEMS Immunol Med Microbiol. 1996 Jun;14(2-3):167-77. doi: 10.1111/j.1574-695X.1996.tb00284.x.
The relative immunogenicity of tetanus toxin fragment C (TTFC) has been determined in three different strains of inbred mice when expressed in Lactococcus lactis as a membrane-anchored protein (strain UCP1054), as an intracellular protein (strain UCP1050), or as a secreted protein which is partly retained within the cell wall (strain UCP1052). Protection against toxin challenge (20 x LD50) could be obtained without the induction of anti-lactococcal antibodies. When compared in terms of the dose of expressed tetanus toxin fragment C required to elicit protection against lethal challenge the membrane-anchored form was significantly (10-20 fold) more immunogenic than the alternative forms of the protein.
已在三种不同品系的近交系小鼠中测定了破伤风毒素片段C（TTFC）的相对免疫原性，该片段在乳酸乳球菌中分别作为膜锚定蛋白（UCP1054菌株）、细胞内蛋白（UCP1050菌株）或部分保留在细胞壁内的分泌蛋白（UCP1052菌株）表达。无需诱导抗乳球菌抗体即可获得针对毒素攻击（20×LD50）的保护。就引发针对致死性攻击的保护所需的表达破伤风毒素片段C的剂量而言，膜锚定形式的免疫原性比该蛋白的其他形式显著高（10至20倍）。
