Rebello C M, Jobe A H, Eisele J W, Ikegami M
Department of Pediatrics, Habor-UCLA Medical Center, UCLA School of Medicine 90509, USA.
Am J Respir Crit Care Med. 1996 Sep;154(3 Pt 1):625-8. doi: 10.1164/ajrccm.154.3.8810596.
The amounts of surfactant in human lung tissue and in the alveolar pool have not been extensively reported. We used 24 human lungs from persons over the age range of 13 mo to 80 yr to investigate whether the amount of saturated phosphatidylcholine (Sat-PC) in the human lung changed with age. Lung lavages also were obtained from 10 lungs at autopsy for measurements of the alveolar Sat-PC and surfactant protein-A (SP-A) concentrations. We found that (mean +/- SEM) lung Sat-PC/body weight (28.4 +/- 2.2 mumol/kg), Sat-PC/lung protein (0.060 +/- 0.005 mumol/mg), and Sat-PC/lung deoxyribonucleic acid (DNA) (0.23 +/- 0.01 mumol/mg) did not vary significantly with age. The amounts of alveolar Sat-PC (1.9 +/- 0.2 mumol/kg) and SP-A (105 +/- 15 micrograms/kg) were lower than previous estimates in other species. The relatively small surfactant pools in the human may make the lung more susceptible to injuries that interfere with surfactant function.
人类肺组织和肺泡池中的表面活性剂含量尚未得到广泛报道。我们使用了24例年龄在13个月至80岁之间的人类肺脏，来研究人类肺中饱和磷脂酰胆碱（Sat-PC）的含量是否随年龄变化。还在尸检时从10个肺脏获取了肺灌洗样本，以测量肺泡Sat-PC和表面活性剂蛋白A（SP-A）的浓度。我们发现，（均值±标准误）肺Sat-PC/体重（28.4±2.2μmol/kg）、Sat-PC/肺蛋白（0.060±0.005μmol/mg）和Sat-PC/肺脱氧核糖核酸（DNA）（0.23±0.01μmol/mg）并不随年龄显著变化。肺泡Sat-PC（1.9±0.2μmol/kg）和SP-A（105±15μg/kg）的含量低于之前对其他物种的估计值。人类相对较小的表面活性剂池可能使肺更容易受到干扰表面活性剂功能的损伤。
