Plewka A, Kamiński M
Department of Histology and Embryology, Silesian School of Medicine, Katowice-Ligota, Poland.
Exp Toxicol Pathol. 1996 Jun;48(4):249-53. doi: 10.1016/S0940-2993(96)80009-1.
The influence of the cholesterol and protein diet on the mixed-function oxidases system activity and desaturation of fatty acids were examined. The phenobarbital innducibility of these parameters was analyzed too. Investigations were carried out on Wistar adult male rats. The animals were on the cholesterol (0.25%) and protein diet (32%) during 45 days. The cytochrome P-450 and cytochrome b5 contents, the NADPH-cytochrome P-450 reductase and NADH-cytochrome b5 reductase activities, aniline hydroxylase and 4-aminopyrine N-demethylase activities were measured in the hepatic microsomal fraction. In both sexes, the cholesterol diet decreased all the examined parameters with the exception of NADH-cytochrome b5 reductase activity. The protein diet did not change the examined enzyme activities or levels with the exception of induction of NADH-cytochrome b5 reductase and 4-aminopyrine N-demethylase activities. Simultaneous treatment with phenobarbital had heterogenous effect dependent on the type of diet and examined parameter.
研究了胆固醇和蛋白质饮食对混合功能氧化酶系统活性及脂肪酸去饱和作用的影响。还分析了这些参数的苯巴比妥诱导性。以成年雄性Wistar大鼠进行实验。动物在含胆固醇（0.25%）和蛋白质（32%）的饮食条件下喂养45天。测定肝微粒体部分的细胞色素P - 450和细胞色素b5含量、NADPH - 细胞色素P - 450还原酶和NADH - 细胞色素b5还原酶活性、苯胺羟化酶和4 - 氨基比林N - 脱甲基酶活性。在两性中，除NADH - 细胞色素b5还原酶活性外，胆固醇饮食降低了所有检测参数。除诱导NADH - 细胞色素b5还原酶和4 - 氨基比林N - 脱甲基酶活性外，蛋白质饮食未改变所检测的酶活性或水平。同时用苯巴比妥处理产生的异质性效应取决于饮食类型和检测参数。
