Clopton RE, Gold RE
Center for Urban Entomology, Texas A&M University, College Station, Texas, 77843-2475
J Invertebr Pathol. 1996 May;67(3):219-23. doi: 10.1006/jipa.1996.0036.
The host specificity of Gregarina blattarum was evaluated among five species of domiciliary cockroaches: Blatella germanica, Supella longipalpa, Blatta orientalis, Periplaneta americana, and Periplaneta fuliginosa. Third- and fourth-instar nymphs were allowed to feed on crushed dog kibble contaminated with G. blattarum oocysts. Cockroaches were killed 8 days postinfection and examined for parasites. Gregarina blattarum infections were observed in all experimentally infected replications of B. germanica. No infection was observed in an experimentally infected replication of S. longipalpa, B. orientalis, P. americana, or P. fuliginosa, nor was an infection observed in a time zero or ending time control group. In vitro excystation assays using extracts of host gut homogenates demonstrate that G. blattarum sporozoites successfully excyst and begin the life cycle in all five cockroach species tested. No excystation was observed in neutral buffered saline controls. These data suggest that G. blattarum comprises a complex of cryptic species marked by narrow host utilization rather than a single species parasitizing a broad array of cockroach taxa.
在五种居家蟑螂中评估了德国裸蠊的宿主特异性，这五种蟑螂分别是：德国小蠊、长翅污斑蠊、东方蜚蠊、美洲大蠊和黑胸大蠊。让三龄和四龄若虫取食被德国裸蠊卵囊污染的碾碎狗粮。感染8天后处死蟑螂并检查是否有寄生虫。在所有实验感染的德国小蠊样本中均观察到德国裸蠊感染。在实验感染的长翅污斑蠊、东方蜚蠊、美洲大蠊或黑胸大蠊样本中未观察到感染，在零时间或结束时间对照组中也未观察到感染。使用宿主肠道匀浆提取物进行的体外脱囊试验表明，德国裸蠊子孢子在所有测试的五种蟑螂物种中均成功脱囊并开始生命周期。在中性缓冲盐水对照组中未观察到脱囊现象。这些数据表明，德国裸蠊由一系列宿主利用范围狭窄的隐存物种组成，而非寄生于多种蟑螂类群的单一物种。
