Meffre E, Fougereau M, Argenson J N, Aubaniac J M, Schiff C
Centre d'Immunologie de Marseille-Luminy (CIML), France.
Eur J Immunol. 1996 Sep;26(9):2172-80. doi: 10.1002/eji.1830260932.
Surrogate light chains (psi L) encoded by lambda-like (lambda 5) and VpreB genes play a critical role in controlling the early steps of B cell differentiation. We prepared new anti-VpreB monoclonal antibodies (mAb) (3C7/6F6) which preferentially recognize the VpreB epitope at the cell surface of human cell lines that do not express the mu chain. These mAb provide the first characterization of human pro-B cell lines expressing surface psi L. We demonstrate that surface psi L expression is considerably enhanced upon interleukin-7 stimulation and that the psi L complex is formed independently of the Ig alpha/Ig beta heterodimer. Finally, using these antibodies, we confirm the existence of a normal pro-B cell population in human adult bone marrow. These cells are CD34+ CD38+ psi L+, do or do not express CD19, CD10, or both epitopes, and may represent the earliest cell population committed to B cell differentiation.
由类λ基因（λ5）和VpreB基因编码的替代轻链（ψL）在控制B细胞分化的早期步骤中起关键作用。我们制备了新的抗VpreB单克隆抗体（mAb）（3C7/6F6），其优先识别不表达μ链的人细胞系细胞表面的VpreB表位。这些单克隆抗体首次对表达表面ψL的人前B细胞系进行了表征。我们证明，白细胞介素-7刺激后表面ψL表达显著增强，且ψL复合物独立于Igα/Igβ异二聚体形成。最后，使用这些抗体，我们证实了人类成人骨髓中存在正常的前B细胞群体。这些细胞为CD34+CD38+ψL+，表达或不表达CD19、CD10或这两个表位，可能代表最早致力于B细胞分化的细胞群体。
