Lamari Y, Boissard C, Moukhtar M S, Jullienne A, Rosselin G, Garel J M
E.A. 1526 DRED, Université Pierre et Marie Curie, Paris, France.
FEBS Lett. 1996 Sep 16;393(2-3):248-52. doi: 10.1016/0014-5793(96)00895-2.
We have characterized, by RT-PCR amplification using specific primers, the presence of glucagon-like peptide-1 (GLP-I) receptor mRNA in CA-77 cells, a C cell line derived from a rat medullary thyroid carcinoma. Down-regulation of the GLP-1 receptor mRNA was observed after exposure of CA-77 C cells with GLP-1 (7-37). Increased secretion of both calcitonin gene-related peptide (CGRP) and calcitonin (CT) occurred after treatment with GLP-1 (7-37) associated with elevated steady-state levels of CGRP and CT mRNA. GLP-1 (7-37) increased cAMP formation in CA-77 cells in a dose-dependent manner; exendin (9-39), a GLP-1 receptor antagonist, inhibited cAMP production. The GLP-1 peptide which is produced by intestinal cells could be involved in the control of CT secretion through an entero-thyroidal axis implying GLP-1 receptor and increased CT gene expression.
我们已通过使用特异性引物进行逆转录聚合酶链反应（RT-PCR）扩增，鉴定了源自大鼠甲状腺髓样癌的C细胞系CA-77细胞中胰高血糖素样肽-1（GLP-1）受体mRNA的存在情况。在用GLP-1（7-37）处理CA-77 C细胞后，观察到GLP-1受体mRNA的下调。用GLP-1（7-37）处理后，降钙素基因相关肽（CGRP）和降钙素（CT）的分泌均增加，同时CGRP和CT mRNA的稳态水平升高。GLP-1（7-37）以剂量依赖性方式增加CA-77细胞中cAMP的生成；GLP-1受体拮抗剂艾塞那肽（9-39）可抑制cAMP的产生。肠道细胞产生的GLP-1肽可能通过肠-甲状腺轴参与CT分泌的调控，这意味着GLP-1受体和CT基因表达增加。
