Kanzaki H, Morishita Y, Akiyama H, Arata J
Department of Dermatology, Okayama University Medical School, Japan.
J Dermatol Sci. 1996 Jun;12(2):132-9. doi: 10.1016/0923-1811(95)00472-6.
Staphylococcus aureus cells attach to and invade the epidermis more easily under conditions of abrasion or occlusion or in the presence of irritant dermatitis than when the epidermis is intact. This fact strongly suggests that exuded plasma components may play an important role in the adherence of S. aureus cells to the horny layer. S. aureus cells (Cowan 1 strain, Wood 46 strain, and the protein A-deficient mutant, C7 strain, which was isolated from the Cowan 1 strain) were epicutaneously inoculated on the backs of mice. Biopsy specimens were taken from the mice at 1 h, 3 h, and 6 h after inoculation and examined using immunoelectron microscopy. Gold particles for fibrinogen gathered in a time-dependent manner at the interfaces of S. aureus cells and horny material in the lesions inoculated with the Cowan 1 and C7 strains but not in the lesions inoculated with the Wood 46 strain. These results suggest that fibrinogen plays a role in the binding of S. aureus cells to the horny layer.
与表皮完整时相比，在磨损、闭塞或存在刺激性皮炎的情况下，金黄色葡萄球菌细胞更容易附着并侵入表皮。这一事实有力地表明，渗出的血浆成分可能在金黄色葡萄球菌细胞与角质层的黏附中起重要作用。将金黄色葡萄球菌细胞（考恩1株、伍德46株以及从考恩1株分离出的蛋白A缺陷突变体C7株）经皮接种到小鼠背部。在接种后1小时、3小时和6小时从小鼠身上采集活检标本，并使用免疫电子显微镜进行检查。在接种考恩1株和C7株的病变部位，用于标记纤维蛋白原的金颗粒以时间依赖性方式聚集在金黄色葡萄球菌细胞与角质物质的界面处，而在接种伍德46株的病变部位则未出现这种情况。这些结果表明，纤维蛋白原在金黄色葡萄球菌细胞与角质层的结合中起作用。
