Young R C, Hession R O
Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston 29425, USA.
J Muscle Res Cell Motil. 1996 Jun;17(3):349-55. doi: 10.1007/BF00240932.
Intracellular calcium waves were observed in cultured human myometrium using confocal laser scanning microscopy in the epifluorescence mode. Intracellular calcium waves were observed following stimulation of the cells with 10 mU ml-1 oxytocin in physiological bathing solution, or following substitution of tetraethylammonium chloride for NaCl. Intracellular wave speeds were 18.9 +/- 8.2 microns s-1 in physiological bathing solution. Intracellular calcium waves were also observed in cells bathed in calcium-free solutions, with no significant differences in wave speeds. Calcium waves that crossed cell boundaries were also observed. Some intercellular calcium waves travelled large distances (> 500 microns). Wave speeds for intercellular waves were 7.8 +/- 1.6 microns s-1. Attempts to use octanol to determine the contribution of gap junctions to intercellular calcium wave propagation were unsuccessful because of direct effects of 2 mM octanol on calcium oscillations. These data demonstrate that intra- and intercellular calcium waves are a means of tissue level communication in myometrium that is distinct from action potentials with regard to mechanisms of action, propagation speeds, responses to stimuli, and responses to inhibitors.
在共聚焦激光扫描显微镜的落射荧光模式下，观察培养的人子宫肌层中的细胞内钙波。在用生理浴液中10 mU/ml的催产素刺激细胞后，或用四乙铵氯化物替代氯化钠后，观察到细胞内钙波。在生理浴液中，细胞内波速为18.9±8.2微米/秒。在无钙溶液中培养的细胞中也观察到细胞内钙波，波速无显著差异。还观察到跨越细胞边界的钙波。一些细胞间钙波传播距离很远（>500微米）。细胞间波的波速为7.8±1.6微米/秒。由于2 mM辛醇对钙振荡有直接影响，因此尝试用辛醇来确定间隙连接对细胞间钙波传播的贡献未成功。这些数据表明，细胞内和细胞间钙波是子宫肌层中组织水平通讯的一种方式，在作用机制、传播速度、对刺激的反应以及对抑制剂的反应方面与动作电位不同。
