Oyama Y, Chikahisa L, Ueha T, Kanemaru K, Noda K
Laboratory of Cell Signaling (Pharmacology), Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Brain Res. 1996 Mar 18;712(2):349-52. doi: 10.1016/0006-8993(95)01440-3.
Effect of Ginkgo biloba extract was examined on dissociated rat cerebellar neurons suffering from oxidative stress induced by hydrogen peroxide using a flow cytometer and ethidium bromide. Hydrogen peroxide at a concentration of 3 mM increased the number of neurons stained with ethidium (presumably dead neurons) in a time-dependent manner. Pretreatment of neurons with G. biloba extract (10 micrograms/ml) greatly delayed a time-dependent increase in number of dead neurons during exposure to hydrogen peroxide. It was true, but less effective, in the case of treatment with G. biloba extract immediately or 60 min after start of oxidative stress. Results implicate G. biloba extract as a potential agent in protecting the neurons suffering from oxidative stress induced by hydrogen peroxide.
使用流式细胞仪和溴化乙锭，研究了银杏叶提取物对过氧化氢诱导氧化应激的离体大鼠小脑神经元的影响。浓度为3 mM的过氧化氢以时间依赖性方式增加了用溴化乙锭染色的神经元数量（可能是死亡神经元）。用银杏叶提取物（10微克/毫升）预处理神经元，可在很大程度上延迟暴露于过氧化氢期间死亡神经元数量的时间依赖性增加。在氧化应激开始后立即或60分钟用银杏叶提取物处理，情况也是如此，但效果较差。结果表明，银杏叶提取物可能是一种保护遭受过氧化氢诱导氧化应激的神经元的潜在药物。
