Ni Y, Zhao B, Hou J, Xin W
Institute of Biophysics, Academia Sinica, Beijing, People's Republic of China.
Neurosci Lett. 1996 Aug 23;214(2-3):115-8. doi: 10.1016/0304-3940(96)12897-4.
The ability of oxidative stress to induce apoptosis, and the effect of Ginkgo biloba extract (EGb761) on this induction were studied in primary cultured rat cerebellar neuronal cells. Cells were exposed to hydroxyl radicals by treating them with 20-50 microM hydrogen peroxide (H2O2) and 100 microM ferrous sulfate. Hydroxyl radical treatment fragmented the DNA in a manner typical of apoptosis cells, producing a ladder pattern of 200 base pair increments on 1% agarose gel electrophoresis. Pretreatment of cells with 100 micrograms/ml EGb reduced hydroxyl radical induced cells apoptosis (determined by flow cytometry) and DNA fragmentation. The results indicate that hydroxyl radicals induce apoptosis in rat cerebellar neuronal cells and this induction can be prevented by EGb.
在原代培养的大鼠小脑神经元细胞中，研究了氧化应激诱导细胞凋亡的能力以及银杏叶提取物（EGb761）对这种诱导作用的影响。通过用20 - 50微摩尔过氧化氢（H2O2）和100微摩尔硫酸亚铁处理细胞，使其暴露于羟自由基中。羟自由基处理以典型的凋亡细胞方式使DNA断裂，在1%琼脂糖凝胶电泳上产生200碱基对增量的梯状条带模式。用100微克/毫升EGb预处理细胞可减少羟自由基诱导的细胞凋亡（通过流式细胞术测定）和DNA断裂。结果表明，羟自由基可诱导大鼠小脑神经元细胞凋亡，而EGb可预防这种诱导作用。
