Hall L M, Whiley R A, Duke B, George R C, Efstratiou A
Department of Medical Microbiology, London Hospital Medical College, United Kingdom.
J Clin Microbiol. 1996 Apr;34(4):853-9. doi: 10.1128/jcm.34.4.853-859.1996.
A collection of 54 isolates of invasive Streptococcus pneumoniae of serotypes 3 and 14 and serogroups 6, 9, 19, and 23 was investigated. Multilocus enzyme electrophoresis and pulsed-field gel electrophoresis suggested that two clones were represented in the collection, one of serotype 14 isolates, most of which were resistant to erythromycin, and one of serotype 9V isolates, in which resistance to penicillin (MIC, 1 microgram/ml), cefotaxime, and co-trimoxazole was common. Among other isolates there were only a limited correlation between genetic relatedness measured by multilocus enzyme electrophoresis and expression of the same capsule type. However, isolates with highly related pulsed-field gel electrophoresis patterns always shared the same serotype and highly related allele profiles. Calculation of the index of association suggests a freely recombining population structure with epidemic spread of successful clones.
对54株血清型3和14以及血清群6、9、19和23的侵袭性肺炎链球菌分离株进行了研究。多位点酶电泳和脉冲场凝胶电泳表明，该集合中有两个克隆，一个是血清型14分离株克隆，其中大多数对红霉素耐药，另一个是血清型9V分离株克隆，其中对青霉素（MIC，1微克/毫升）、头孢噻肟和复方新诺明耐药很常见。在其他分离株中，通过多位点酶电泳测量的遗传相关性与相同荚膜类型的表达之间只有有限的相关性。然而，具有高度相关脉冲场凝胶电泳图谱的分离株总是具有相同的血清型和高度相关的等位基因谱。关联指数的计算表明，该群体结构具有自由重组的特征，成功克隆以流行方式传播。
