Versalovic J, Kapur V, Mason E O, Shah U, Koeuth T, Lupski J R, Musser J M
Dept. of Pathology, Baylor College of Medicine, Houston, TX 77030.
J Infect Dis. 1993 Apr;167(4):850-6. doi: 10.1093/infdis/167.4.850.
A sample of 48 penicillin-resistant Streptococcus pneumoniae (PRSP) strains recovered between January 1989 and May 1991, primarily from infected children in Houston, was characterized by multilocus enzyme electrophoresis and repetitive extragenic palindromic-polymerase chain reaction genomic profiling. A heterogeneous array of 22 clonal genotypes was identified, but 64% of the PRSP strains in the sample were assigned to five clones that are closely similar in overall chromosomal character and express serotype 6 capsule. A close genetic association between these five clones and penicillin-resistant 6B clones recovered in Alaska, Iceland, and Spain was identified by multilocus enzyme electrophoresis. Taken together, the results suggest either that the common resistant 6B clones in Alaska, Iceland, Spain, and Houston have a recent ancestor or that isolates of a certain pneumococcal phylogenetic lineage are more likely to develop penicillin resistance.
1989年1月至1991年5月期间收集的48株耐青霉素肺炎链球菌（PRSP）菌株样本，主要来自休斯顿受感染儿童，通过多位点酶电泳和重复外显子回文序列-聚合酶链反应基因组分析进行特征鉴定。鉴定出22种不同的克隆基因型，但样本中64%的PRSP菌株被归为五个克隆，这些克隆在整体染色体特征上非常相似，并表达6型荚膜。通过多位点酶电泳确定了这五个克隆与在阿拉斯加、冰岛和西班牙分离出的耐青霉素6B克隆之间存在密切的遗传关联。综合来看，结果表明要么阿拉斯加、冰岛、西班牙和休斯顿常见的耐药6B克隆有一个近期的共同祖先，要么特定肺炎链球菌系统发育谱系的分离株更有可能产生青霉素耐药性。
