Selman M, Ramos C, Montaño M, Montfort I, Perez-Tamayo R
División de Investigación Clínica, Instituto Nacional de Enfermedades Respiratorias, Secretaría de Salud, México City.
Arch Biol Med Exp. 1987;20(3-4):311-7.
We have studied the effects of soluble factors obtained from rat lungs with experimentally-induced pulmonary fibrosis of 2 months duration on the in vitro rates of biosynthesis and degradation of collagen in normal rat lung preparations. Factors soluble in phosphate-buffered saline were prepared from the minced lungs of normal controls and of silicotic animals. The in vitro rate of collagen biosynthesis of normal rat lung explants was measured as the rate of incorporation of radioactive proline into total and collagenous protein. The in vitro rate of collagen degradation in normal rat lung homogenates was measured as the rate of release of hydroxyproline-containing materials of less than 100,000 daltons to the supernatant. Our results suggest that in this experimental model of pulmonary fibrosis there are soluble factors that stimulate both collagen biosynthesis and collagen degradation in in vitro preparations of normal rat lung.
我们研究了从患有持续2个月实验性肺纤维化的大鼠肺中获得的可溶性因子对正常大鼠肺组织中胶原蛋白体外生物合成和降解速率的影响。从正常对照大鼠和矽肺大鼠的切碎肺组织中制备磷酸盐缓冲盐溶液可溶的因子。正常大鼠肺外植体中胶原蛋白的体外生物合成速率通过放射性脯氨酸掺入总蛋白和胶原蛋白的速率来测定。正常大鼠肺匀浆中胶原蛋白的体外降解速率通过分子量小于100,000道尔顿的含羟脯氨酸物质释放到上清液中的速率来测定。我们的结果表明，在这个肺纤维化实验模型中，存在可刺激正常大鼠肺体外制剂中胶原蛋白生物合成和胶原蛋白降解的可溶性因子。
