Mennuni C, Santini C, Dotta F, Farilla L, Di Mario U, Fierabracci A, Bottazzo G, Cortese R, Luzzago A
Istituto di Ricerche di Biologia Molecolare P. Angeletti (IRBM), Pomezia, Rome, Italy.
J Autoimmun. 1996 Jun;9(3):431-6. doi: 10.1006/jaut.1996.0060.
Phage display technology represents a powerful tool for the identification of peptides reacting with disease-related antibodies present in human sera. The application of this technology to type 1 diabetes could provide a set of novel reagents for diabetes prediction and could also lead to the identification of novel autoantigens or even of environmental factors possibly causing the disease. In the present study, sera of prediabetic and high risk individuals were used to select candidate peptides from phage-displayed random peptide libraries. Diabetes specific phage clones were then identified from these through screening and counter screening, using sera from diabetic and non-diabetic individuals. The results presented in this paper demonstrate the feasibility of this methodology to identify peptides reacting preferentially with antibodies present in the serum of diabetic patients.
噬菌体展示技术是一种强大的工具，可用于鉴定与人类血清中存在的疾病相关抗体发生反应的肽段。将该技术应用于1型糖尿病，可为糖尿病预测提供一套新型试剂，还可能导致鉴定出新的自身抗原，甚至可能鉴定出可能引发该疾病的环境因素。在本研究中，利用糖尿病前期和高风险个体的血清从噬菌体展示的随机肽库中筛选候选肽段。然后，通过使用糖尿病患者和非糖尿病患者的血清进行筛选和反向筛选，从这些肽段中鉴定出糖尿病特异性噬菌体克隆。本文给出的结果证明了该方法用于鉴定与糖尿病患者血清中存在的抗体优先发生反应的肽段的可行性。
