Martínez-Abad M, Estañ L, Martínez-Mir I, Rubio E, Morales-Olivas F J
Department of Surgery, Hospital Dr. Peset, Valencia, Spain.
Methods Find Exp Clin Pharmacol. 1996 Jun;18(5):327-33.
The spontaneous activity of isolated human colon strips was studied to obtain homogeneous results with a reproducible model. The strips of macroscopically normal appearance were mounted in an organ bath containing Krebs solution at 32 degrees C or Tyrode solution at 37 degrees C. Mechanical activity was recorded by an isometric transducer. Spontaneous motility did not occur in all preparations. Moreover, when observed, it could not always be evaluated. The percentage of strips with spontaneous activity was lower with Tyrode solution than with Krebs solution (65 vs. 81%). KC1 did not induce a plateau contraction. Acetylcholine induced concentration-dependent contractions, with a significantly different pD2: 4.43 +/- 0.39 and 5.59 +/- 0.16 for Krebs and Tyrode, respectively. Isoprenaline abolished spontaneous motility in Krebs solution. Only 20% of specimens presented evaluable motility. Krebs solution may be the best conditions for studying the effects of drugs on spontaneous motility, while Tyrode solution can be used to investigate the effects of contractile agents.
为了通过可重复的模型获得一致的结果，对分离的人结肠条的自发活动进行了研究。外观宏观正常的结肠条被置于含有32℃的 Krebs 溶液或37℃的 Tyrode 溶液的器官浴中。通过等长换能器记录机械活动。并非所有制剂中都会出现自发运动。此外，当观察到自发运动时，也并非总能进行评估。使用 Tyrode 溶液时，具有自发活动的结肠条百分比低于使用 Krebs 溶液时（分别为65%和81%）。氯化钾不会诱导平台收缩。乙酰胆碱诱导浓度依赖性收缩，Krebs 溶液和 Tyrode 溶液的 pD2 有显著差异，分别为4.43±0.39和5.59±0.16。异丙肾上腺素消除了 Krebs 溶液中的自发运动。只有20%的标本呈现可评估的运动。Krebs 溶液可能是研究药物对自发运动影响的最佳条件，而 Tyrode 溶液可用于研究收缩剂的作用。
