Sakaguchi M, Nakamura H, Sonoda K, Hamada F, Hirai K
Chemo-Sero Therapeutic Research Institute, Kikuchi Research Center, Kumamoto, Japan.
Vaccine. 1996 Jun;14(8):747-52. doi: 10.1016/0264-410x(95)00254-x.
To evaluate the usefulness of a DNA vaccine for chickens, we constructed a plasmid vector expressing the Newcastle disease virus F protein (NDV-F) under the control of the human cytomegalovirus immediate early enhancer and chicken beta-actin gene promoter. One-week-old chickens injected intramusculary with the circular plasmid DNA did not produce significant levels of antibody against NDV-F. However, two of five birds injected with the linearized plasmid DNA produced high levels of the antibody. Moreover, four of five birds injected with a mixture of the linearized-plasmid and Lipofectin produced the antibody efficiently. At 9 weeks post-injection, chickens were challenged with the velogenic NDV Sato strain. These chickens that had the antibody against NDV-F were protected from lethal NDV challenge. These results demonstrate that the DNA vaccine conferred efficient protection against the disease.
为评估一种鸡用DNA疫苗的效用，我们构建了一种质粒载体，该载体在人巨细胞病毒立即早期增强子和鸡β-肌动蛋白基因启动子的控制下表达新城疫病毒F蛋白（NDV-F）。一周龄的鸡肌肉注射环状质粒DNA后，未产生显著水平的抗NDV-F抗体。然而，五只注射线性化质粒DNA的鸡中有两只产生了高水平的抗体。此外，五只注射线性化质粒和脂质体混合物的鸡中有四只高效产生了抗体。注射后9周，用强毒株新城疫病毒佐藤株攻击鸡。这些具有抗NDV-F抗体的鸡受到保护，免受致死性新城疫病毒攻击。这些结果表明，该DNA疫苗对该疾病具有有效的保护作用。
