Cameron R B, Watts J C, Kasten B L
Am J Clin Pathol. 1979 Jul;72(1):90-3. doi: 10.1093/ajcp/72.1.90.
Laboratory procedures used to establish the diagnosis of Pneumocystis carinii pneumonia were evaluated using an experimental murine model. Touch preparations and suspension smears were prepared from lung tissue know to contain Pneumocystis cysts. These preparations were stained by a variety of methods known to demonstrate either cyst forms or sporozoites and trophozoites. Suspension smears proved to be superior to touch preparations in terms of cyst content and homogeneity of staining. Also, methods that stain cyst forms were superior to those that stain sporozoites and trophozoites for location and identification of organisms. The authors believe that suspension smears prepared from lung tissue and stained with toluidine blue O should be examined initially as a rapid screening method for Pneumocystis cysts. When the results of this initial screen are negative or inconclusive, additional suspension smears stainded by the modified Gomori methenamine silver nitrate method should be examined, pending availability of histologic sections.
使用实验性小鼠模型评估了用于确立卡氏肺孢子虫肺炎诊断的实验室程序。从已知含有卡氏肺孢子虫囊肿的肺组织制备触片和悬液涂片。这些涂片通过多种已知可显示囊肿形态或子孢子及滋养体的方法进行染色。就囊肿含量和染色均匀性而言，悬液涂片被证明优于触片。此外，对于生物体的定位和识别，染色囊肿形态的方法优于染色子孢子和滋养体的方法。作者认为，应首先检查用甲苯胺蓝O染色的肺组织悬液涂片，作为卡氏肺孢子虫囊肿的快速筛查方法。当该初步筛查结果为阴性或不确定时，在获得组织学切片之前，应检查用改良的Gomori六胺银硝酸盐法染色的额外悬液涂片。
