Kang S W, Hyung S M, Choi M Y, Lee J
Department of Ophthalmology, College of Medicine, Chungbuk National University, Korea.
Korean J Ophthalmol. 1995 Dec;9(2):69-78. doi: 10.3341/kjo.1995.9.2.69.
To simulate the posterior vitreous detachment (PVD) in the rabbit, 1 IU hyaluronidase and/or 0.2 ml of perfluoropropane gas was intravitreally injected. Ophthalmoscopic, light microscopic examination prepared by cryotechnique, electron microscopic examination, and electroretinogram were done on the 3rd and 28th postoperative days. As a result, the eyes undergone simultaneous intravitreal injection of 1 IU hyaluronidase and 0.2 ml perfluoropropane gas showed membranous structure split from the internal limiting membrane of the superior retina in 3 days after injection. The eyes also demonstrated membranous structure separated from the superior retina after 28 days, simulating vitreous detachment. On the contrary, neither agent alone induced vitreous detachment. No toxic retinal changes associated with simultaneous intravitreal injection of 1 IU hyaluronidase and 0.2 ml perfluoropropane gas were observed. Therefore, with a future support by histologic examination other than cryotechnique and by immunohistochemical analysis, the simultaneous intravitreal injection of perfluoropropane gas and hyaluronidase would be a promising method to induce vitreous detachment in non-vitrectomized eye.
为模拟兔眼的玻璃体后脱离（PVD），经玻璃体腔内注射1国际单位透明质酸酶和/或0.2毫升全氟丙烷气体。在术后第3天和第28天进行检眼镜检查、采用冷冻技术制备的光学显微镜检查、电子显微镜检查以及视网膜电图检查。结果显示，在注射后3天，同时经玻璃体腔内注射1国际单位透明质酸酶和0.2毫升全氟丙烷气体的眼，可见从视网膜上半部分的内界膜分离出的膜状结构。在28天后，这些眼也显示出从视网膜上半部分分离出的膜状结构，模拟了玻璃体脱离。相反，单独使用任何一种药物均未诱导玻璃体脱离。未观察到与同时经玻璃体腔内注射1国际单位透明质酸酶和0.2毫升全氟丙烷气体相关的毒性视网膜改变。因此，在未来除冷冻技术外通过组织学检查和免疫组织化学分析的支持下，同时经玻璃体腔内注射全氟丙烷气体和透明质酸酶将是一种在非玻璃体切除眼中诱导玻璃体脱离的有前景的方法。
