特定性类固醇在大鼠肝脏中形成DNA加合物的情况。

Formation of DNA-adducts by selected sex steroids in rat liver.

Feser W, Kerdar R S, Blode H, Reimann R

Research Laboratories, Schering Aktiengesellschaft, Berlin, Germany.

Hum Exp Toxicol. 1996 Jul;15(7):556-62. doi: 10.1177/096032719601500702.

Abstract

We are reporting investigations into the potential of the steroid hormones chlormadinone acetate (CMA), cyproterone acetate (CPA), ethinylestradiol (EE2) gestodene (GEST), megestrol acetate (MGA), norethisterone acetate (NET-Ac), estradiol (E2), and progesterone (P) to form DNA-adducts in rat liver in vivo. 2. Compound-related DNA-adduct spots were detected in male and female rat liver following CMA, CPA, and MGA using the 32P-postlabeling-technique. Substance-specific DNA-adducts were also observed in male rats after administration of E2. The other compounds showed no DNA-adduct formation. After treatment with CMA, CPA or MGA, the relative adduct labeling (RAL) differed sex- and substance-specifically.

摘要

我们正在报告关于甾体激素醋酸氯地孕酮（CMA）、醋酸环丙孕酮（CPA）、炔雌醇（EE2）、孕二烯酮（GEST）、醋酸甲地孕酮（MGA）、醋酸炔诺酮（NET-Ac）、雌二醇（E2）和孕酮（P）在大鼠肝脏中体内形成DNA加合物潜力的研究。2. 使用32P后标记技术，在给予CMA、CPA和MGA后，在雄性和雌性大鼠肝脏中检测到与化合物相关的DNA加合物斑点。给予E2后，在雄性大鼠中也观察到物质特异性的DNA加合物。其他化合物未显示出DNA加合物的形成。用CMA、CPA或MGA处理后，相对加合物标记（RAL）因性别和物质而异。