Fong T L, Lee S R, Briggs W K, Valinluck B, Govindarajan S, Hoffman A, Jaczko B, Redeker A G
Division of Gastrointestinal and Liver Diseases, University of Southern California School of Medicine, Los Angeles, USA.
J Med Virol. 1996 Jul;49(3):253-8. doi: 10.1002/(SICI)1096-9071(199607)49:3<253::AID-JMV16>3.0.CO;2-D.
Extensive serological testing and HCV RNA determination by RT-PCR was performed in serum, PBMCs, and liver tissue in thirteen anti-HCV reactive patients with persistently normal liver tests. Absolute concordance in the status of HCV RNA between serum, PBMCs, and liver was noted. Five patients were HCV RNA positive but only three had mild histological changes. Eight patients were HCV RNA negative in all three sites and had virtually normal liver histology. Patterns of reactivity in RIBA 2.0 strip immunoblot assay did not differentiate viremic from nonviremic patients. ELISA testing using multiple individual HCV recombinant antigens from the structural and non-structural regions of HCV demonstrated mean antibody titers to the structural antigens, in particular HCV E2 antibodies, to be significantly lower in HCV RNA negative patients. The status of HCV RNA in the serum appears to infer the status of HCV RNA in the liver and PBMCs in patients with persistently normal liver tests. Patients with persistently normal liver tests and undetectable HCV RNA have probably spontaneously cleared HCV infection.
对13例抗-HCV反应性患者进行了广泛的血清学检测，并通过RT-PCR在血清、外周血单个核细胞（PBMC）和肝组织中测定HCV RNA，这些患者的肝功能检查持续正常。血清、PBMC和肝脏之间HCV RNA状态完全一致。5例患者HCV RNA阳性，但只有3例有轻度组织学改变。8例患者在所有三个部位HCV RNA均为阴性，肝脏组织学基本正常。重组免疫印迹法（RIBA）2.0条带免疫印迹分析的反应模式无法区分病毒血症患者和非病毒血症患者。使用来自HCV结构和非结构区域的多种单个HCV重组抗原进行ELISA检测显示，HCV RNA阴性患者对结构抗原的平均抗体滴度，特别是HCV E2抗体滴度显著降低。在肝功能检查持续正常的患者中，血清中HCV RNA的状态似乎可以推断肝脏和PBMC中HCV RNA的状态。肝功能检查持续正常且HCV RNA检测不到的患者可能已自发清除HCV感染。
