Mathai A, Radhakrishnan V V, Sehgal S
Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum.
Indian J Pathol Microbiol. 1995 Jul;38(3):299-303.
Antibodies to two mycobacterial antigens viz - culture filtrate antigen (CFA) and Mycobacterium tuberculosis antigen 5 were raised in rabbits. Enzyme-linked immuno sorbent assay (ELISA) and immunoblot methods were used for the evaluation of the specificity of the rabbit antibodies to M. tuberculosis. Immunoblot method is more sensitive than ELISA for the detection of antibodies to M. tuberculosis in the rabbit sera. It is being emphasised that characterisation of the mycobacterial antigens and evaluation of the specificity of the antimycobacterial antibodies are essential prior to their applications as an adjunct in the laboratory diagnosis of human mycobacterial disease.
针对两种分枝杆菌抗原，即培养滤液抗原（CFA）和结核分枝杆菌抗原5，在兔体内产生了抗体。采用酶联免疫吸附测定（ELISA）和免疫印迹法评估兔抗结核分枝杆菌抗体的特异性。在检测兔血清中结核分枝杆菌抗体方面，免疫印迹法比ELISA更敏感。需要强调的是，在将分枝杆菌抗原及其抗分枝杆菌抗体作为辅助手段应用于人类分枝杆菌病的实验室诊断之前，对其进行表征和评估其特异性至关重要。
