Radhakrishnan V V, Mathai A, Rao S B, Sehgal S
Division of Pathology, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum, India.
Indian J Exp Biol. 1990 Sep;28(9):812-5.
Polyvalent antiserum to culture filtrate of H37 Ra M. tuberculosis was raised in rabbits. Monospecific antiserum was raised against M. tuberculosis antigen-5, prepared from the culture filtrates by immunoabsorbent affinity chromatography. On immunoelectrophoresis, antigen-5 demonstrated single precipitin arc against polyvalent and monospecific antisera. The culture filtrate antigen demonstrated multiple precipitin arcs against polyvalent antiserum and single precipitin are against monospecific antiserum. Antigen-5 could be isolated and characterized from the culture filtrate of H37 Ra M. tuberculosis. Immunoelectrophoresis could be one of the method to characterize the mycobacterial antigens prepared in the laboratory.
用结核分枝杆菌H37 Ra的培养滤液在兔体内制备了多价抗血清。通过免疫吸附亲和色谱法从培养滤液中制备了针对结核分枝杆菌抗原-5的单特异性抗血清。在免疫电泳中，抗原-5与多价和单特异性抗血清均显示出单一沉淀弧。培养滤液抗原与多价抗血清显示出多条沉淀弧，与单特异性抗血清显示出单一沉淀弧。抗原-5可以从结核分枝杆菌H37 Ra的培养滤液中分离并进行表征。免疫电泳可能是表征实验室制备的分枝杆菌抗原的方法之一。
