Gormand F, Cheria-Sammari S, Aloui R, Guibert B, Malicier D, Perrin-Fayolle M, Lagarde M, Pacheco Y
Unité INSERM 352, INSA, Villeurbanne, France.
Pulm Pharmacol. 1995 Apr-Jun;8(2-3):107-13. doi: 10.1006/pulp.1995.1013.
The bronchial epithelium produces cytokines that could contribute to inflammatory events in airways. In this study we determined the basal and TNFalpha stimulated productions of GM-CSF and IL-8 by human bronchial epithelial cells (HBEC) collected from 12 control and six asthmatic patients. Spontaneous and TNFalpha-induced GM-CSF or IL-8 released levels increased significantly with time. Epithelial cells from asthmatic patients spontaneously released high levels of GM-CSF (24 h). TNFalpha potentiated GM-CSF and IL-8 release in control subjects and only the IL-8 production in asthmatics. Nedocromil sodium, an antiinflammatory drug, and salbutamol, a beta2-agonist, are commonly used in asthma. They were evaluated on the spontaneous and TNF-induced expression of GM-CSF and IL-8 in cultured bronchial epithelial cells. Nedocromil sodium, at the concentration of 10(-6) M, reduced the TNF-induced increase in GM-CSF but not the IL-8 release. Salbutamol, at the concentration of 10(-6) or 10(-5) M, did not affect the constitutive or stimulated production of both cytokines.
支气管上皮细胞可产生细胞因子，这些细胞因子可能会引发气道炎症反应。在本研究中，我们测定了从12名对照者和6名哮喘患者采集的人支气管上皮细胞（HBEC）中GM-CSF和IL-8的基础分泌水平以及经肿瘤坏死因子α（TNFα）刺激后的分泌水平。GM-CSF和IL-8的自发分泌水平以及经TNFα诱导后的释放水平均随时间显著增加。哮喘患者的上皮细胞自发分泌高水平的GM-CSF（24小时）。TNFα可增强对照者中GM-CSF和IL-8的释放，而在哮喘患者中仅增强IL-8的分泌。色甘酸钠是一种抗炎药物，沙丁胺醇是一种β2受体激动剂，常用于治疗哮喘。我们评估了它们对培养的支气管上皮细胞中GM-CSF和IL-8的自发表达以及TNF诱导表达的影响。浓度为10^(-6) M的色甘酸钠可降低TNF诱导的GM-CSF增加，但不影响IL-8的释放。浓度为10^(-6) 或10^(-5) M的沙丁胺醇对这两种细胞因子的基础分泌或刺激分泌均无影响。
