Suzuki H, Katori Y, Takasaka T
Department of Otolaryngology, Tohoku University School of Medicine, Sendai, Japan.
Acta Otolaryngol. 1996 Jan;116(1):12-6. doi: 10.3109/00016489609137706.
The carbohydrate composition of the stereociliary glycocalyx of the utricle was analysed quantitatively. The utricular sensory epithelium was collected from adult albino guinea pigs, and its apical surface structure was blotted onto a PVDF membrane, which was then subjected to acid hydrolysis. The hydrolysate was treated with 1-phenyl-3-methyl-5-pyrazolone for labelling and analysed by reversed-phase HPLC coupled with detection of UV absorbance at 245 nm. Man, GlcN, GaIN, Glc, Gal and Fuc were detected and estimated to be 211, 219, 46, 1,270, 266 and 36 pmoles per 10 utricular maculae, respectively. The presence of Man indicates the presence of N-linked glycoconjugates, and the considerable amounts of GLcN and Gal suggest the presence of complex-type N-glycosides, poly-lactosamine and/or keratan sulfate. The relatively low GaIN content indicates that O-glycosides, chondroitin sulfates and GaINAc-containing glycosphingolipids, i.e. gangliosides and globosides, are minor components. Electronmicroscopic and confocal laser scanning microscopic observations revealed that the blotted apical surface structure constituted mostly the ciliary bundle. Consequently, the observed carbohydrate composition is probably that of the stereociliary glycocalyx of the utricular sensory epithelium.
对椭圆囊静纤毛糖萼的碳水化合物组成进行了定量分析。从成年白化豚鼠收集椭圆囊感觉上皮，将其顶端表面结构印迹到聚偏二氟乙烯（PVDF）膜上，然后进行酸水解。水解产物用1-苯基-3-甲基-5-吡唑啉酮处理进行标记，并通过反相高效液相色谱结合245nm紫外吸光度检测进行分析。检测到甘露糖（Man）、氨基葡萄糖（GlcN）、N-乙酰半乳糖胺（GaIN）、葡萄糖（Glc）、半乳糖（Gal）和岩藻糖（Fuc），估计每10个椭圆囊斑中分别为211、219、46、1270、266和36皮摩尔。甘露糖的存在表明存在N-连接糖缀合物，大量的氨基葡萄糖和半乳糖表明存在复合型N-糖苷、多乳糖胺和/或硫酸角质素。相对较低的N-乙酰半乳糖胺含量表明O-糖苷、硫酸软骨素和含N-乙酰半乳糖胺的糖鞘脂，即神经节苷脂和球苷脂，是次要成分。电子显微镜和共聚焦激光扫描显微镜观察显示，印迹的顶端表面结构主要由纤毛束组成。因此，观察到的碳水化合物组成可能是椭圆囊感觉上皮静纤毛糖萼的组成。
