血红素与谷胱甘肽相互作用的光谱和干涉测量研究。

Spectral and interferometrical study of the interaction of haemin with glutathione.

作者信息

Sahini V E, Dumitrescu M, Volanschi E, Birla L, Diaconu C

机构信息

Department of Physical Chemistry, University of Bucharest, Romania.

出版信息

Biophys Chem. 1996 Feb 8;58(3):245-53. doi: 10.1016/0301-4622(95)00110-7.

DOI:10.1016/0301-4622(95)00110-7

PMID:8820409

Abstract

The interaction of haemin with reduced glutathione (GSH) was investigated in vitro and the association constant was determined by both spectrophotometric and interferometric methods. In order to elucidate the nature of this interaction, the reaction of haemin with a typical reductive agent (sodium dithionite) and a typical chelating agent for hem-iron (histidine) was also studied. Haemin-induced haemolysis in the presence and absence of GSH was monitored spectrophotometrically. The results outline the role of GSH at physiological concentration in preventing erythrocyte damage by haemin.

摘要

在体外研究了血红素与还原型谷胱甘肽（GSH）的相互作用，并通过分光光度法和干涉测量法测定了缔合常数。为了阐明这种相互作用的性质，还研究了血红素与典型的还原剂（连二亚硫酸钠）和血红素铁的典型螯合剂（组氨酸）的反应。用分光光度法监测了在有和没有GSH的情况下血红素诱导的溶血作用。结果概述了生理浓度的GSH在防止血红素对红细胞造成损伤方面的作用。