Ekins S
Department of Medicine, University of Aberdeen, Indianapolis, IN 46285, USA.
Drug Metab Dispos. 1996 Mar;24(3):364-6.
Testosterone and 7-ethoxycoumarin were used as substrates to quantify the maintenance of phase I and II enzymes in precision-cut rat liver slices in dynamic organ culture. Testosterone hydroxylations, 7-ethoxycoumarin O-deethylation, 7-hydroxycoumarin sulfate, and 7-hydroxycoumarin glucuronide formation were all maintained at initial levels in the slice incubation media after incubation for up to 4 hr. The activities of various cytochrome P450 isozymes, measured using the stereospecific and regiospecific hydroxylation of testosterone and the maintenance of phase I and II metabolism using 7-ethoxycoumarin, are therefore suggested to be stable over short-term incubations in a physiological buffer. The testosterone hydroxylation assay is also suggested as a versatile slice metabolic viability marker for various P450 activities over longer periods.
睾酮和7-乙氧基香豆素被用作底物，以量化动态器官培养中精密切割的大鼠肝切片中I相和II相酶的维持情况。在长达4小时的孵育后，睾酮羟化、7-乙氧基香豆素O-脱乙基、7-羟基香豆素硫酸盐和7-羟基香豆素葡糖醛酸的形成在切片孵育培养基中均维持在初始水平。因此，使用睾酮的立体特异性和区域特异性羟化以及使用7-乙氧基香豆素维持I相和II相代谢来测量的各种细胞色素P450同工酶的活性，在生理缓冲液中短期孵育期间被认为是稳定的。睾酮羟化测定法也被认为是一种通用的切片代谢活力标志物，可用于更长时间内各种P450活性的检测。
