Ogawa T, Nakazawa M, Masui K
Department of Oral Microbiology, Osaka University, Japan.
Vaccine. 1996 Jan;14(1):70-76. doi: 10.1016/0264-410x(95)00128-n.
The low endotoxic lipid A derived from Porphyromonas gingivalis 381, 1-phospho beta(1-6)-linked glucosamine disaccharide with 3-hydroxy-15-methylhexadecanoyl and 3-hexadecanoyloxy-15-methylhexadecanoyl groups at the 2- and 2'-positions, respectively, induced mitogenic responses in LPS low responder C3H/HeJ as well as LPS responder C3H/HeN mouse splenocytes. The mitogenic activities of P. gingivalis lipid A in splenocytes of LPS responder mice were comparable to that of the synthetic Escherichia coli-type lipid A (compound 506). The addition of polymyxin B resulted in the inhibition of mitogenic activity. P. gingivalis lipid A also stimulated strongly nonspecific host resistance against Pseudomonas aeruginosa infection in BALB/c mice, and specific immune response in guinea pigs against infection with P. gingivalis. Furthermore, P. gingivalis lipid A demonstrated antitumour activity against MH134 hepatoma in C3H/HeN mice. These life-preserving with tumour regression properties were comparable to those of monophosphoryl lipid A derived from Salmonella minnesota Re 595. Thus, P. gingivalis lipid A appears to have beneficial properties as an immunopharmacological agent.
源自牙龈卟啉单胞菌381的低内毒素脂质A，即1-磷酸-β(1-6)-连接的葡糖胺二糖，在2-位和2'-位分别带有3-羟基-15-甲基十六烷酰基和3-十六烷酰氧基-15-甲基十六烷酰基，可在LPS低反应性C3H/HeJ以及LPS反应性C3H/HeN小鼠脾细胞中诱导促有丝分裂反应。牙龈卟啉单胞菌脂质A在LPS反应性小鼠脾细胞中的促有丝分裂活性与合成的大肠杆菌型脂质A（化合物506）相当。加入多粘菌素B会导致促有丝分裂活性受到抑制。牙龈卟啉单胞菌脂质A还能强烈刺激BALB/c小鼠对铜绿假单胞菌感染的非特异性宿主抵抗力，以及豚鼠对牙龈卟啉单胞菌感染的特异性免疫反应。此外，牙龈卟啉单胞菌脂质A对C3H/HeN小鼠的MH134肝癌具有抗肿瘤活性。这些具有肿瘤消退特性的保护生命的特性与源自明尼苏达沙门氏菌Re 595的单磷酰脂质A相当。因此，牙龈卟啉单胞菌脂质A似乎具有作为免疫药理剂的有益特性。
