Ogawa T
Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
FEBS Lett. 1993 Oct 11;332(1-2):197-201. doi: 10.1016/0014-5793(93)80512-s.
The novel chemical structure of Porphyromonas (Bacteroides) gingivalis strain 381 lipid A was determined to be a glucosamine beta-(1-6) disaccharide 1-monophosphate acylated by 3-hydroxy-15-methylhexadecanoic acid and 3-hexadecanoyloxy-15-methylhexadecanoic acid at the 2- and 2'-positions, respectively. The absence of ester-linked phosphate at the 4'-position and fatty acids at the 3- and 3'-positions, and the presence of fatty acids possessing 16-17 carbon atoms are unique features, differentiating the lipid A from enterobacterial and other lipid As. These structural features may be related to its low endotoxic activity. Furthermore, P. gingivalis lipid A as well as its LPS stimulated the splenocytes from C3H/HeN and C3H/HeJ mice.
牙龈卟啉单胞菌（拟杆菌）菌株381脂多糖A的新型化学结构被确定为一种β-(1-6)连接的葡糖胺二糖1-单磷酸，在2位和2'位分别被3-羟基-15-甲基十六烷酸和3-十六烷酰氧基-15-甲基十六烷酸酰化。4'位不存在酯键连接的磷酸基团，3位和3'位不存在脂肪酸，以及存在具有16-17个碳原子的脂肪酸是其独特特征，使该脂多糖A与肠杆菌属脂多糖A及其他脂多糖A区分开来。这些结构特征可能与其低内毒素活性有关。此外，牙龈卟啉单胞菌脂多糖A及其脂多糖刺激了C3H/HeN和C3H/HeJ小鼠的脾细胞。
