Yu H S, Chang K L, Yu C L, Chen J W, Chen G S
Department of Dermatology, Kaohsiung Medical College, Taiwan, Republic of China.
J Invest Dermatol. 1996 Oct;107(4):593-6. doi: 10.1111/1523-1747.ep12583090.
Clinical observations have suggested that low-energy lasers might promote wound healing. Evidence suggests that He-Ne laser irradiation induces an increase in the rate of keratinocyte migration and proliferation as compared with nonirradiated controls in vitro. This study sought to determine whether He-Ne laser could induce cytokine production in cultured keratinocytes. The results revealed (i) a significant increase in interleukin-1 alpha and interleukin-8 production and their respective mRNA expression in He-Ne laser-treated groups as compared with nonirradiated controls, and (ii) under 1.5 joules/cm2 irradiation, this stimulating effect of He-Ne laser treatment is concentration-dependent. Because interleukin-1 alpha induces keratinocyte migration, this finding may partially explain the stimulatory effects on the motility of keratinocytes. As both interleukin-1 alpha and interleukin-8 provoke proliferation of keratinocytes, it is not unreasonable to propose that these two cytokines play a profound role in the enhancement of keratinocyte proliferation as a result of He-Ne laser irradiation. Our findings provide further evidence of enhanced wound healing at the cellular and molecular level as a result of the He-Ne laser.
临床观察表明，低能量激光可能促进伤口愈合。有证据显示，与体外未照射的对照组相比，氦氖激光照射可诱导角质形成细胞迁移和增殖速率增加。本研究旨在确定氦氖激光是否能诱导培养的角质形成细胞产生细胞因子。结果显示：（i）与未照射的对照组相比，氦氖激光治疗组中白细胞介素-1α和白细胞介素-8的产生及其各自的mRNA表达显著增加；（ii）在1.5焦耳/平方厘米的照射剂量下，氦氖激光治疗的这种刺激作用具有浓度依赖性。由于白细胞介素-1α可诱导角质形成细胞迁移，这一发现可能部分解释了对角质形成细胞运动性的刺激作用。鉴于白细胞介素-1α和白细胞介素-8均可刺激角质形成细胞增殖，因此可以合理推测，这两种细胞因子在氦氖激光照射导致的角质形成细胞增殖增强过程中发挥着重要作用。我们的研究结果进一步证明了氦氖激光在细胞和分子水平上促进伤口愈合的作用。
