Müller-Ladner U, Gross V, Andus T, Gschwendtner H, Roth M, Caesar I, Schölmerich J, Lang B
Department of Internal Medicine I, University of Regensburg, Germany.
Eur J Gastroenterol Hepatol. 1996 Jun;8(6):579-84. doi: 10.1097/00042737-199606000-00016.
The pathophysiological significance of autoantibodies in inflammatory bowel diseases (IBD) is still unclear. To assess specific immunological abnormalities we examined the distribution and restriction of immunoglobulin classes and subclasses of anti-neutrophil cytoplasmic antibodies (ANCAs) in ulcerative colitis and of antibodies to pancreatic juice (APJs) in Crohn's disease.
We tested 62 sera of patients with ulcerative colitis and 184 sera of patients with Crohn's disease for their immunoglobulin class and IgG subclass distribution of ANCAs (in ulcerative colitis patients) and APJs (in Crohn's disease patients) by fluorescence isothiocyanate (FITC)-labelled anti-human antibodies in an immunofluorescence assay with human granulocytes or pancreas as substrate. Twenty-five patients with Wegener's granulomatosis were used for comparison.
In ulcerative colitis ANCAs were found in 74% of the patients. They were predominantly of the IgG (96%) and IgA class (37%). In Crohn's disease APJs (present in 28% of the cases) were of the IgG (98%) and IgA class (71%). ANCAs in ulcerative colitis were of the IgG1 (73%) and IgG3 (5%) subclasses, APJs in Crohn's disease of the IgG1 (94%), IgG2 (20%) and IgG3 (2%) subclasses. The immunoglobulin class and IgG subclass distributions of ANCAs and APJs resembled the changes in total immunoglobulin classes and IgG subclasses in ulcerative colitis or Crohn's disease.
The immunoglobulin classes and IgG subclasses of autoantibodies in ulcerative colitis and Crohn's disease differ from each other and from the distribution of autoantibodies in vasculitic diseases (Wegener's granulomatosis, microscopic vasculitis, systemic lupus erythematosus). These alterations reflect specific pathophysiological features of ulcerative colitis and Crohn's disease.
自身抗体在炎症性肠病（IBD）中的病理生理意义仍不清楚。为评估特定的免疫异常，我们检测了溃疡性结肠炎中抗中性粒细胞胞浆抗体（ANCA）的免疫球蛋白类别和亚类的分布及限制性，以及克罗恩病中抗胰液抗体（APJ）的情况。
我们通过以人粒细胞或胰腺为底物的免疫荧光测定法，用异硫氰酸荧光素（FITC）标记的抗人抗体检测了62例溃疡性结肠炎患者的血清和184例克罗恩病患者的血清中ANCA（针对溃疡性结肠炎患者）和APJ（针对克罗恩病患者）的免疫球蛋白类别及IgG亚类分布。选取25例韦格纳肉芽肿患者作为对照。
在溃疡性结肠炎患者中，74%的患者检测到ANCA。它们主要为IgG类（96%）和IgA类（37%）。在克罗恩病中，28%的病例检测到APJ，其为IgG类（98%）和IgA类（71%）。溃疡性结肠炎中的ANCA为IgG1亚类（73%）和IgG3亚类（5%），克罗恩病中的APJ为IgG1亚类（94%）、IgG2亚类（20%）和IgG3亚类（2%）。ANCA和APJ的免疫球蛋白类别及IgG亚类分布类似于溃疡性结肠炎或克罗恩病中总免疫球蛋白类别及IgG亚类的变化。
溃疡性结肠炎和克罗恩病中自身抗体的免疫球蛋白类别及IgG亚类彼此不同，且与血管炎性疾病（韦格纳肉芽肿、显微镜下血管炎、系统性红斑狼疮）中自身抗体的分布不同。这些改变反映了溃疡性结肠炎和克罗恩病特定的病理生理特征。
