Layes E, Schollmayer A, Spessert R, Vollrath L
Department of Anatomy, Johannes Gutenberg-University, Mainz, Germany.
Neurosci Lett. 1996 Jul 5;212(1):71-3. doi: 10.1016/0304-3940(96)12765-8.
In the developing rat pineal gland, norepinephrine (NE)-stimulated cyclic guanosine 3',5'-monophosphate (cGMP) formation appears 2 weeks later than that of cyclic adenosine 3',5'-monophosphate (cAMP) formation. Since NE-stimulated cGMP formation requires formation of nitric oxide (NO) in the adult rat pineal, we investigated the developmental appearance of the NO-forming enzyme brain NO-synthase (NOS) to find out whether the delayed cGMP response results from the late appearance of NOS. In this study, we investigated pineal glands of rats aged 8-21 days and 8 weeks. Western blot analysis revealed that NOS-immunoreactivity (NOS-IR) was barely detectable until day 12. On day 15 NOS-IR exhibited the same intensity as in adult rats. Expression of NOS at this postnatal stage appears to be responsible for the developmental appearance of the adrenergic cGMP response.
在发育中的大鼠松果体中，去甲肾上腺素（NE）刺激的环磷酸鸟苷（cGMP）生成比环磷酸腺苷（cAMP）生成晚两周出现。由于在成年大鼠松果体中，NE刺激的cGMP生成需要一氧化氮（NO）的生成，我们研究了生成NO的酶——脑一氧化氮合酶（NOS）的发育情况，以确定cGMP反应延迟是否是由于NOS出现较晚所致。在本研究中，我们调查了8 - 21天龄和8周龄大鼠的松果体。蛋白质免疫印迹分析显示，直到第12天，NOS免疫反应性（NOS-IR）几乎检测不到。在第15天，NOS-IR的强度与成年大鼠相同。出生后这个阶段NOS的表达似乎是肾上腺素能cGMP反应发育出现的原因。
