Müthing J
Institute of Cell Culture Technology, University of Bielefeld, Germany.
Carbohydr Res. 1996 Sep 2;290(2):217-24. doi: 10.1016/0008-6215(96)00149-8.
Influenza A and Sendai viruses are known to bind to various extent to neolacto-series gangliosides IV3Neu5Ac-nLcOse4Cer, IV6Neu5Ac-nLcOse4Cer, and VI3Neu5Ac-nLcOse6Cer, which are the dominant gangliosides of human granulocytes. Recently, minor gangliosides of granulocytes were characterized and found to express sialyl Lewis(x) and VIM-2 epitopes. These long chain linear monosialogangliosides with nLcOse8, and nLcOse10, cores, carrying one to three fucoses, are shown in this study to bind with strong avidity to influenza A/PR/8/34 (H1N1), A/X-31 (H3N2), and Sendai virus (Z-strain) using the overlay technique. These and recent data from other groups imply that selectins and virus hemagglutinins are capable of competing with lipid bound sialyl Lewis(x) and VIM-2 epitopes on myeloid cells during inflammatory reactions.
已知甲型流感病毒和仙台病毒能不同程度地结合新乳糖系列神经节苷脂IV3Neu5Ac-nLcOse4Cer、IV6Neu5Ac-nLcOse4Cer和VI3Neu5Ac-nLcOse6Cer，这些是人类粒细胞的主要神经节苷脂。最近，粒细胞的次要神经节苷脂被鉴定出来，并发现其表达唾液酸化路易斯（x）和VIM-2表位。本研究表明，这些具有nLcOse8和nLcOse10核心、携带一至三个岩藻糖的长链线性单唾液酸神经节苷脂，使用覆盖技术能与甲型流感病毒A/PR/8/34（H1N1）、A/X-31（H3N2）和仙台病毒（Z株）强烈结合。这些以及其他研究小组最近的数据表明，在炎症反应期间，选择素和病毒血凝素能够与髓样细胞上脂质结合的唾液酸化路易斯（x）和VIM-2表位竞争。
