Müthing J, Unland F, Heitmann D, Orlich M, Hanisch F G, Peter-Katalinić J, Knäuper V, Tschesche H, Kelm S, Schauer R
Institut für Zellkulturtechnik, Universität Bielefeld, Germany.
Glycoconj J. 1993 Feb;10(1):120-6. doi: 10.1007/BF00731196.
The structures of gangliosides from human granulocytes were elucidated by fast atom bombardment mass spectrometry and by gas chromatography/mass spectrometry as their partially methylated alditol acetates. In human granulocytes besides GM3 (II3Neu5Ac-LacCer), neolacto-series gangliosides (IV3Neu5Ac-nLcOse4Cer, IV6Neu5Ac-nLcOse4Cer and VI3Neu5Ac-nLcOse6Cer) containing C24:1, and to some extent C22:0; and C16:0 fatty acid in their respective ceramide portions, were identified as major components. In this study we demonstrate that gangliosides from human granulocytes, the second most abundant cells in peripheral blood, can serve as receptors for influenza viruses A/PR/8/34 (H1N1), A/X-31 (H3N2), and a parainfluenza virus Sendai virus (HNF1, Z-strain). Viruses were found to exhibit specific adhesion to terminal Neu5Ac alpha 2-3Gal and/or Neu5Ac alpha 2-6Gal sequences as well as depending on the chain length of ganglioside carbohydrate backbones from human granulocytes, these important effector cells which represent the first line of defence in immunologically mediated reactions.
通过快原子轰击质谱法以及气相色谱/质谱法，以其部分甲基化的糖醇乙酸酯形式，阐明了人粒细胞中神经节苷脂的结构。在人粒细胞中，除了GM3（II3Neu5Ac-LacCer）外，还鉴定出了新乳糖系列神经节苷脂（IV3Neu5Ac-nLcOse4Cer、IV6Neu5Ac-nLcOse4Cer和VI3Neu5Ac-nLcOse6Cer），其神经酰胺部分含有C24:1，在一定程度上还含有C22:0和C16:0脂肪酸，这些是主要成分。在本研究中，我们证明，人粒细胞（外周血中第二丰富的细胞）中的神经节苷脂可作为甲型流感病毒A/PR/8/34（H1N1）、A/X-31（H3N2）以及副流感病毒仙台病毒（HNF1，Z株）的受体。发现病毒对末端Neu5Acα2-3Gal和/或Neu5Acα2-6Gal序列表现出特异性粘附，并且取决于人粒细胞神经节苷脂碳水化合物主链的链长，这些重要的效应细胞代表了免疫介导反应中的第一道防线。
