弗氏柠檬酸杆菌中依赖辅酶B12的甘油脱水酶编码基因的克隆、测序及过表达
Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii.
作者信息
Seyfried M, Daniel R, Gottschalk G
机构信息
Institut fur Mikrobiologie der Georg-August-Universitat, Gottingen, Germany.
出版信息
J Bacteriol. 1996 Oct;178(19):5793-6. doi: 10.1128/jb.178.19.5793-5796.1996.
Abstract
The genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii were cloned and overexpressed in Escherichia coli. The B12-free enzyme was purified to homogeneity. It consists of three types of subunits whose N-terminal sequences are in accordance with those deduced from the open reading frames dhaB, dhaC, and dhaE, coding for subunits of 60,433 (alpha), 21,487 (beta), and 16,121 (gamma) Da, respectively. The enzyme complex has the composition alpha2beta2gamma2. Amino acid alignments with the subunits of the recently sequenced diol dehydratase of Klebsiella oxytoca (T. Tobimatsu, T. Hara, M. Sakaguchi, Y. Kishimoto, Y. Wada, M. Isoda, T. Sakai, and T. Toraya, J. Biol. Chem. 270:7142-7148, 1995) revealed identities between 51.8 and 70.9%.
摘要
弗氏柠檬酸杆菌中编码依赖辅酶B12的甘油脱水酶的基因被克隆并在大肠杆菌中过表达。不含B12的酶被纯化至同质。它由三种类型的亚基组成，其N端序列与从开放阅读框dhaB、dhaC和dhaE推导的序列一致，分别编码60433（α）、21487（β）和16121（γ）Da的亚基。酶复合物的组成为α2β2γ2。与最近测序的产酸克雷伯菌二醇脱水酶的亚基进行氨基酸比对（T. Tobimatsu、T. Hara、M. Sakaguchi、Y. Kishimoto、Y. Wada、M. Isoda、T. Sakai和T. Toraya，《生物化学杂志》270:7142 - 7148，1995年），发现同源性在51.8%至70.9%之间。
相似文献
1
Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii.
J Bacteriol. 1996 Oct;178(19):5793-6. doi: 10.1128/jb.178.19.5793-5796.1996.
2
Identification and expression of the genes and purification and characterization of the gene products involved in reactivation of coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii.
Eur J Biochem. 2001 Apr;268(8):2369-78. doi: 10.1046/j.1432-1327.2001.02123.x.
3
Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum.
FEMS Microbiol Lett. 1998 Jul 1;164(1):21-8. doi: 10.1111/j.1574-6968.1998.tb13062.x.
4
Molecular cloning, co-expression, and characterization of glycerol dehydratase and 1,3-propanediol dehydrogenase from Citrobacter freundii.
Mol Biotechnol. 2013 Jun;54(2):469-74. doi: 10.1007/s12033-012-9585-9.
5
[Construction of novel recombinant strain harboring glycerol dehydratase reactivating factor capable of producing 1,3-propanediol].
Sheng Wu Gong Cheng Xue Bao. 2007 Sep;23(5):841-5.
6
Identification and characterization of coenzyme B12-dependent glycerol dehydratase- and diol dehydratase-encoding genes from metagenomic DNA libraries derived from enrichment cultures.
Appl Environ Microbiol. 2003 Jun;69(6):3048-60. doi: 10.1128/AEM.69.6.3048-3060.2003.
7
Growth temperature-dependent activity of glycerol dehydratase in Escherichia coli expressing the Citrobacter freundii dha regulon.
FEMS Microbiol Lett. 1992 Dec 15;100(1-3):281-5. doi: 10.1111/j.1574-6968.1992.tb14053.x.
8
The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol.
Eur J Biochem. 2002 Sep;269(18):4484-94. doi: 10.1046/j.1432-1033.2002.03151.x.
9
Molecular cloning, sequencing and characterization of the genes for adenosylcobalamin-dependent diol dehydratase of Klebsiella pneumoniae.
Biosci Biotechnol Biochem. 1998 Sep;62(9):1774-7. doi: 10.1271/bbb.62.1774.
10
Purification of 1,3-propanediol dehydrogenase from Citrobacter freundii and cloning, sequencing, and overexpression of the corresponding gene in Escherichia coli.
J Bacteriol. 1995 Apr;177(8):2151-6. doi: 10.1128/jb.177.8.2151-2156.1995.
引用本文的文献
1
Evidence for corrin biosynthesis in the last universal common ancestor.
FEBS J. 2025 Feb;292(4):827-850. doi: 10.1111/febs.17367. Epub 2024 Dec 21.
2
Metabolism, morphology and transcriptome analysis of oscillatory behavior of Clostridium butyricum during long-term continuous fermentation for 1,3-propanediol production.
Biotechnol Biofuels. 2020 Nov 25;13(1):191. doi: 10.1186/s13068-020-01831-8.
3
Key enzymes catalyzing glycerol to 1,3-propanediol.
Biotechnol Biofuels. 2016 Mar 10;9:57. doi: 10.1186/s13068-016-0473-6. eCollection 2016.
4
Molecular cloning, co-expression, and characterization of glycerol dehydratase and 1,3-propanediol dehydrogenase from Citrobacter freundii.
Mol Biotechnol. 2013 Jun;54(2):469-74. doi: 10.1007/s12033-012-9585-9.
5
Inactivation of dhaD and dhaK abolishes by-product accumulation during 1,3-propanediol production in Klebsiella pneumoniae.
J Ind Microbiol Biotechnol. 2010 Jul;37(7):707-16. doi: 10.1007/s10295-010-0714-9. Epub 2010 Apr 9.
6
Identification and characterization of coenzyme B12-dependent glycerol dehydratase- and diol dehydratase-encoding genes from metagenomic DNA libraries derived from enrichment cultures.
Appl Environ Microbiol. 2003 Jun;69(6):3048-60. doi: 10.1128/AEM.69.6.3048-3060.2003.
7
Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum.
Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5010-5. doi: 10.1073/pnas.0734105100. Epub 2003 Apr 18.
8
Construction and screening of metagenomic libraries derived from enrichment cultures: generation of a gene bank for genes conferring alcohol oxidoreductase activity on Escherichia coli.
Appl Environ Microbiol. 2003 Mar;69(3):1408-16. doi: 10.1128/AEM.69.3.1408-1416.2003.
9
Regulation of carbon and electron flow in Clostridium butyricum VPI 3266 grown on glucose-glycerol mixtures.
J Bacteriol. 2001 Mar;183(5):1748-54. doi: 10.1128/JB.183.5.1748-1754.2001.
10
The propanediol utilization (pdu) operon of Salmonella enterica serovar Typhimurium LT2 includes genes necessary for formation of polyhedral organelles involved in coenzyme B(12)-dependent 1, 2-propanediol degradation.
J Bacteriol. 1999 Oct;181(19):5967-75. doi: 10.1128/JB.181.19.5967-5975.1999.
本文引用的文献
1
Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri.
Appl Environ Microbiol. 1990 Apr;56(4):1195-7. doi: 10.1128/aem.56.4.1195-1197.1990.
2
A cobamide-requiring glycerol dehydrase from an acrolein-forming Lactobacillus.
Arch Biochem Biophys. 1962 Jun;97:538-43. doi: 10.1016/0003-9861(62)90118-2.
3
Molecular weight estimations of proteins by electrophoresis in polyacrylamide gels of graded porosity.
FEBS Lett. 1972 Feb 1;20(2):199-202. doi: 10.1016/0014-5793(72)80793-2.
4
How a protein binds B12: A 3.0 A X-ray structure of B12-binding domains of methionine synthase.
Science. 1994 Dec 9;266(5191):1669-74. doi: 10.1126/science.7992050.
5
Purification of 1,3-propanediol dehydrogenase from Citrobacter freundii and cloning, sequencing, and overexpression of the corresponding gene in Escherichia coli.
J Bacteriol. 1995 Apr;177(8):2151-6. doi: 10.1128/jb.177.8.2151-2156.1995.
6
Molecular cloning, sequencing, and expression of the genes encoding adenosylcobalamin-dependent diol dehydrase of Klebsiella oxytoca.
J Biol Chem. 1995 Mar 31;270(13):7142-8. doi: 10.1074/jbc.270.13.7142.
7
Biochemical and molecular characterization of the oxidative branch of glycerol utilization by Citrobacter freundii.
J Bacteriol. 1995 Aug;177(15):4392-401. doi: 10.1128/jb.177.15.4392-4401.1995.
8
Distribution of coenzyme B12-dependent diol dehydratase and glycerol dehydratase in selected genera of Enterobacteriaceae and Propionibacteriaceae.
J Bacteriol. 1980 Mar;141(3):1439-42. doi: 10.1128/jb.141.3.1439-1442.1980.
9
DHA system mediating aerobic and anaerobic dissimilation of glycerol in Klebsiella pneumoniae NCIB 418.
J Bacteriol. 1982 Aug;151(2):591-9. doi: 10.1128/jb.151.2.591-599.1982.
10
Purification and properties of dihydroxyacetone kinase from Klebsiella pneumoniae.
J Bacteriol. 1984 Oct;160(1):55-60. doi: 10.1128/jb.160.1.55-60.1984.
Zotero 插件