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Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri.鼠李糖乳杆菌甘油脱水酶的纯化和性质研究。
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Purification of 1,3-propanediol dehydrogenase from Citrobacter freundii and cloning, sequencing, and overexpression of the corresponding gene in Escherichia coli.弗氏柠檬酸杆菌1,3 - 丙二醇脱氢酶的纯化及其相应基因在大肠杆菌中的克隆、测序与过表达
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Molecular cloning, sequencing, and expression of the genes encoding adenosylcobalamin-dependent diol dehydrase of Klebsiella oxytoca.产酸克雷伯菌中腺苷钴胺素依赖性二醇脱水酶编码基因的分子克隆、测序及表达
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Biochemical and molecular characterization of the oxidative branch of glycerol utilization by Citrobacter freundii.弗氏柠檬酸杆菌甘油利用氧化分支的生化与分子特征
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弗氏柠檬酸杆菌中依赖辅酶B12的甘油脱水酶编码基因的克隆、测序及过表达

Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii.

作者信息

Seyfried M, Daniel R, Gottschalk G

机构信息

Institut fur Mikrobiologie der Georg-August-Universitat, Gottingen, Germany.

出版信息

J Bacteriol. 1996 Oct;178(19):5793-6. doi: 10.1128/jb.178.19.5793-5796.1996.

DOI:10.1128/jb.178.19.5793-5796.1996
PMID:8824629
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC178423/
Abstract

The genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii were cloned and overexpressed in Escherichia coli. The B12-free enzyme was purified to homogeneity. It consists of three types of subunits whose N-terminal sequences are in accordance with those deduced from the open reading frames dhaB, dhaC, and dhaE, coding for subunits of 60,433 (alpha), 21,487 (beta), and 16,121 (gamma) Da, respectively. The enzyme complex has the composition alpha2beta2gamma2. Amino acid alignments with the subunits of the recently sequenced diol dehydratase of Klebsiella oxytoca (T. Tobimatsu, T. Hara, M. Sakaguchi, Y. Kishimoto, Y. Wada, M. Isoda, T. Sakai, and T. Toraya, J. Biol. Chem. 270:7142-7148, 1995) revealed identities between 51.8 and 70.9%.

摘要

弗氏柠檬酸杆菌中编码依赖辅酶B12的甘油脱水酶的基因被克隆并在大肠杆菌中过表达。不含B12的酶被纯化至同质。它由三种类型的亚基组成，其N端序列与从开放阅读框dhaB、dhaC和dhaE推导的序列一致，分别编码60433（α）、21487（β）和16121（γ）Da的亚基。酶复合物的组成为α2β2γ2。与最近测序的产酸克雷伯菌二醇脱水酶的亚基进行氨基酸比对（T. Tobimatsu、T. Hara、M. Sakaguchi、Y. Kishimoto、Y. Wada、M. Isoda、T. Sakai和T. Toraya，《生物化学杂志》270:7142 - 7148，1995年），发现同源性在51.8%至70.9%之间。