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利用枯草芽孢杆菌sacB基因进行反选择在黄色粘球菌中产生的pil基因无标记缺失。

Markerless deletions of pil genes in Myxococcus xanthus generated by counterselection with the Bacillus subtilis sacB gene.

作者信息

Wu S S, Kaiser D

机构信息

Department of Biochemistry, Stanford University School of Medicine, California 94305, USA.

出版信息

J Bacteriol. 1996 Oct;178(19):5817-21. doi: 10.1128/jb.178.19.5817-5821.1996.

DOI:10.1128/jb.178.19.5817-5821.1996
PMID:8824635
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC178429/
Abstract

In-frame deletions of pilA and pilS were constructed in Myxococcus xanthus with a plasmid integration-excision strategy facilitated by sacB. sacB conferred sucrose sensitivity upon its M. xanthus host only when it lay in the same orientation as adjacent M. xanthus genes. Gene orientation also affected the efficiency of sucrose counterselection in the sucrose-sensitive strains. The deltapilA mutant lacked pili and social motility, while the deltapilS mutant showed no defect in either phenotype.

摘要

利用由sacB介导的质粒整合-切除策略，在黄色粘球菌中构建了pilA和pilS的框内缺失突变体。仅当sacB与相邻的黄色粘球菌基因处于相同方向时，它才会赋予其黄色粘球菌宿主蔗糖敏感性。基因方向也影响了蔗糖敏感菌株中蔗糖反选择的效率。ΔpilA突变体缺乏菌毛和群体运动性，而ΔpilS突变体在这两种表型上均无缺陷。

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本文引用的文献

1
Developmental cell interactions in Myxococcus xanthus and the spoC locus.粘细菌 Myxococcus xanthus 中的发育细胞相互作用和 spoC 基因座。
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1406-10. doi: 10.1073/pnas.80.5.1406.
2
Cell-to-cell stimulation of movement in nonmotile mutants of Myxococcus.粘球菌非运动突变体中细胞间运动刺激
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2938-42. doi: 10.1073/pnas.74.7.2938.
3
Genetic and functional evidence that Type IV pili are required for social gliding motility in Myxococcus xanthus.遗传和功能证据表明，IV型菌毛是黄色粘球菌社会滑行运动所必需的。
Mol Microbiol. 1995 Nov;18(3):547-58. doi: 10.1111/j.1365-2958.1995.mmi_18030547.x.
4
Dual function of PilS during transcriptional activation of the Pseudomonas aeruginosa pilin subunit gene.铜绿假单胞菌菌毛蛋白亚基基因转录激活过程中PilS的双重功能。
J Bacteriol. 1996 Feb;178(3):831-9. doi: 10.1128/jb.178.3.831-839.1996.
5
Nitrogen control in bacteria.细菌中的氮控制
Microbiol Rev. 1995 Dec;59(4):604-22. doi: 10.1128/mr.59.4.604-622.1995.
6
Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria.通用自杀载体，可直接用于革兰氏阴性菌中基因替换的筛选。
Gene. 1993 May 15;127(1):15-21. doi: 10.1016/0378-1119(93)90611-6.
7
The Agrobacterium tumefaciens virB4 gene product is an essential virulence protein requiring an intact nucleoside triphosphate-binding domain.根癌土壤杆菌virB4基因产物是一种必需的毒力蛋白，需要完整的核苷三磷酸结合结构域。
J Bacteriol. 1993 Mar;175(6):1723-34. doi: 10.1128/jb.175.6.1723-1734.1993.
8
Use of the Vibrio cholerae irgA gene as a locus for insertion and expression of heterologous antigens in cholera vaccine strains.霍乱弧菌irgA基因作为霍乱疫苗菌株中异源抗原插入和表达位点的应用。
Vaccine. 1993 Oct;11(13):1327-35. doi: 10.1016/0264-410x(93)90103-5.
9
A counterselectable pACYC184-based lacZ alpha-complementing plasmid vector with novel multiple cloning sites; construction of chromosomal deletions in Klebsiella pneumoniae.一种基于pACYC184的具有新型多克隆位点的可反向选择的lacZα互补质粒载体；肺炎克雷伯菌染色体缺失的构建
Gene. 1993 Dec 22;136(1-2):253-5. doi: 10.1016/0378-1119(93)90474-h.
10
PilS and PilR, a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa.PilS和PilR，一种控制铜绿假单胞菌IV型菌毛表达的双组分转录调控系统。
Mol Microbiol. 1993 Mar;7(5):669-82. doi: 10.1111/j.1365-2958.1993.tb01158.x.