Kren V, Krenová D, Pravenec M, Zdobinská M
Department of Biology, Charles University, Praha, Czech Republic.
Folia Biol (Praha). 1995;41(6):284-93.
Congenic strains with the polydactyly-luxate syndrome (PLS), the BN.lx and Lew.lx, were originally derived to study the expression and mode of inheritance of the lx mutant gene on rat chromosome 8. The BN.lx PLS congenic strain together with the spontaneously hypertensive SHR strain served as progenitors for the production of the HXB/BXH recombinant inbred (RI) strains. One of the RI strains, the BXH11, carrying PLS was used for the transfer of PLS determining lx allele onto the SHR strain genetic background. Using PCR analysis, differential segments of chromosome 8 of BN.lx and SHR.lx congenic strains were described and shown to carry also genes associated with blood pressure and plasma triglyceride regulation. Possible experimental exploitation of chromosome 8 congenic strains in these respects is thus discussed.
具有多指-脱臼综合征（PLS）的同源近交系，即BN.lx和Lew.lx，最初是为了研究大鼠8号染色体上lx突变基因的表达和遗传模式而培育的。BN.lx PLS同源近交系与自发性高血压SHR品系一起作为产生HXB/BXH重组近交（RI）品系的亲代。其中一个携带PLS的RI品系BXH11被用于将决定PLS的lx等位基因转移到SHR品系的遗传背景上。通过PCR分析，描述了BN.lx和SHR.lx同源近交系8号染色体的差异片段，并表明这些片段还携带与血压和血浆甘油三酯调节相关的基因。因此，讨论了在这些方面对8号染色体同源近交系进行可能的实验利用。
