Moriwaki Y, Yamamoto T, Yamaguchi K, Takahashi S, Higashino K
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
Histochem Cell Biol. 1996 Jan;105(1):71-9. doi: 10.1007/BF01450880.
Tissues from male Wistar rats, fixed with 4% paraformaldehyde and embedded in paraffin, were studied with immunoperoxidase techniques using polyclonal antibodies raised against aldehyde oxidase or xanthine oxidase purified from rat liver. Immunohistochemical studies demonstrated that aldehyde oxidase-bearing cells were strongly stained in renal tubules, esophageal, gastric, intestinal and bronchial epithelium as well as liver cytoplasm. Weak but positive immunoreactivity was observed on the pulmonary alveolar epithelial cells, gastric glands and intestinal goblet cells. In contrast, it was demonstrated that cells with xanthine oxidase were strongly stained in renal tubules, esophageal, gastric, and small and large intestinal and bronchial epithelia etc. Positive immunostaining was also found in adrenal gland, skeletal muscle, spleen and cerebral hippocampus. Immunoreactivity againt aldehyde oxidase was not found in adrenal gland, spleen, mesentery or aorta, while immunoreactivity against xanthine oxidase was not found in mesentery or aorta. Although the significance of this ubiquitous and similar localization of aldehyde and xanthine oxidase seems unclear at present, these results may provide a clue as to the full understanding of the pathophysiological role of these oxidases in tissues.
对用4%多聚甲醛固定并石蜡包埋的雄性Wistar大鼠组织，采用针对从大鼠肝脏纯化的醛氧化酶或黄嘌呤氧化酶制备的多克隆抗体，通过免疫过氧化物酶技术进行研究。免疫组织化学研究表明，含醛氧化酶的细胞在肾小管、食管、胃、肠和支气管上皮以及肝细胞质中被强烈染色。在肺泡上皮细胞、胃腺和肠杯状细胞中观察到弱阳性但阳性的免疫反应性。相比之下，结果表明含黄嘌呤氧化酶的细胞在肾小管、食管、胃、小肠和大肠以及支气管上皮等部位被强烈染色。在肾上腺、骨骼肌、脾脏和脑海马中也发现了阳性免疫染色。在肾上腺、脾脏、肠系膜或主动脉中未发现针对醛氧化酶的免疫反应性，而在肠系膜或主动脉中未发现针对黄嘌呤氧化酶的免疫反应性。尽管目前醛氧化酶和黄嘌呤氧化酶这种普遍且相似定位的意义似乎尚不清楚，但这些结果可能为全面了解这些氧化酶在组织中的病理生理作用提供线索。
