Moriwaki Y, Yamamoto T, Yamakita J, Takahashi S, Higashino K
Third Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan.
Histochem J. 1998 Feb;30(2):69-74. doi: 10.1023/a:1003222831002.
The distribution of aldehyde oxidase activity was evaluated in unfixed cryostat sections from tissues of male Wistar rats using a tissue protectant, polyvinyl alcohol, with Tetranitro BT as a final electron acceptor. The distribution of aldehyde oxidase activity was compared with that of xanthine oxidoreductase. The enzyme histochemical method demonstrated aldehyde oxidase activity in the epithelium of the tongue, renal tubules and bronchioles, as well as in the cytoplasm of liver cells. Such activity was not detected in oesophagus, stomach, spleen, adrenal glands, small or large intestine or skeletal and heart muscle fibres. In contrast, xanthine oxidoreductase activity was demonstrated in the tongue, renal tubules, bronchioles, oesophageal, gastric, small and large intestinal epithelial cells, adrenal glands, spleen and liver cytoplasm but not in skeletal and heart muscle fibres. The significance of the ubiquitous distribution of aldehyde oxidase activity, especially in surface epithelial cells from various tissues, except for the gastrointestinal tract, is unclear. However, aldehyde oxidase may possess some physiological activity other than in the metabolism of N-heterocyclics or of certain drugs.
使用组织保护剂聚乙烯醇，并以四硝基蓝四唑作为最终电子受体，在雄性Wistar大鼠组织的未固定低温切片中评估醛氧化酶活性的分布。将醛氧化酶活性的分布与黄嘌呤氧化还原酶的分布进行比较。酶组织化学方法显示，醛氧化酶活性存在于舌上皮、肾小管和细支气管以及肝细胞的细胞质中。在食管、胃、脾、肾上腺、小肠或大肠以及骨骼肌和心肌纤维中未检测到这种活性。相比之下，黄嘌呤氧化还原酶活性在舌、肾小管、细支气管、食管、胃、小肠和大肠上皮细胞、肾上腺、脾和肝细胞质中显示，但在骨骼肌和心肌纤维中未显示。醛氧化酶活性普遍分布的意义尚不清楚，尤其是在除胃肠道外的各种组织的表面上皮细胞中。然而，醛氧化酶可能具有除参与N-杂环化合物或某些药物代谢之外的一些生理活性。
