通过聚合酶链反应对幽门螺杆菌感染进行实验室诊断。

Laboratory diagnosis of Helicobacter pylori infection by polymerase chain reaction.

作者信息

Ichikawa Y, Mitsuhashi M, Ishikawa T, Wyle F, Chang K, Fujiwara Y, Arakawa T, Shimada H, Tarnawski A

机构信息

VA. Medical Center, Long Beach, CA, USA.

出版信息

Res Commun Mol Pathol Pharmacol. 1996 Jan;91(1):117-28.

PMID:8824937

Abstract

Although the detection of Helicobacter pylori (Hp) infection is important, it is a rather difficult task using only conventional diagnostic techniques. In this study, we have applied a polymerase chain reaction (PCR)-based assay for the diagnosis of Hp infection. RT-PCR for Hp ribosomal RNA was utilized, using the gastric biopsy specimens obtained endoscopically from 10 patients. PCR primers have been designed from the highly consensus region of the 7 different Hp rRNA sequences in GenBank. In the detection of Hp, PCR was more sensitive than culturing and showed 100% of the sensitivity and specificity compared with histology. Our primer pair could detect as few as 50 organisms per reaction. RT-PCR for Hp rRNA is a sensitive and specific method to detect Hp infection in the gastric mucosa.

摘要

虽然检测幽门螺杆菌（Hp）感染很重要，但仅使用传统诊断技术是一项相当困难的任务。在本研究中，我们应用了基于聚合酶链反应（PCR）的检测方法来诊断Hp感染。利用从10例患者内镜下获取的胃活检标本进行Hp核糖体RNA的逆转录聚合酶链反应（RT-PCR）。根据GenBank中7种不同Hp rRNA序列的高度保守区域设计了PCR引物。在检测Hp时，PCR比培养更敏感，与组织学检查相比，其敏感性和特异性均为100%。我们的引物对每次反应能检测低至50个菌。用于Hp rRNA的RT-PCR是检测胃黏膜中Hp感染的一种敏感且特异的方法。