Nicolas-Bolnet C, Qureshi M A, Cieszynski J A, Taylor R L
Department of Poultry Science, North Carolina State University, Raleigh, 27695-7608, USA.
Poult Sci. 1995 Dec;74(12):1970-6. doi: 10.3382/ps.0741970.
The objective of this study was to examine the hematopoietic cell proliferation and differentiation potential of growth factors produced by chicken macrophages. Bone marrow (BM) cells (25 x 10(3)) from newly hatched B15B15 K-strain Leghorn chicks were seeded in .5 mL serum-free semi-solid culture supplemented with 10% (vol/vol) of a conditioned medium (CM) from a chicken macrophage cell line, MQ-NCSU. The conditioned medium was obtained by culturing MQ-NCSU cells either in LM-HAHN (CMI) or RPMI-1640 (CMII) growth medium. The control cultures contained only LM-HAHN or RPMI medium. Bone marrow cells in the presence of CMI differentiated predominately into granulocyte colonies (Experiment 1 = 84 +/- 9.2; Experiment 2 = 105 +/- 5). No colonies were observed in the control cultures. Stimulation of MQ-NCSU cells with lipopolysaccharide (LPS) produced a CM that differentiated BM cells predominantly into macrophage colonies (122 +/- 16.3 in CMI and 92 +/- 5.6 in CMII). These data suggest that MQ-NCSU cells spontaneously secrete a factor with the potential to promote granulocyte differentiation. However, upon stimulation with LPS, the factor secreted had macrophage colony stimulation potential (M-CSF), which was similar in activity when compared with the activity of recombinant chicken myelomonocytic growth factor (r-cMGF). Another CM from chicken fibroblasts (FCM) was tested on BM cells from K-strain Leghorns and Arbor Acres x Arbor Acres broiler chicks. Data from three experiments showed that 25 x 10(3) BM cells from K-strain chicken yielded more macrophage and granulocytes colonies (82 +/- 14) than those from broilers (56 +/- 12). This study suggests that avian cytokines exhibit progenitor cell differentiation potential and that this activity is dependent upon the source of cytokines and their targets.
本研究的目的是检测鸡巨噬细胞产生的生长因子对造血细胞增殖和分化的潜能。将刚孵出的B15B15 K系来亨鸡的骨髓（BM）细胞（25×10³）接种于0.5 mL无血清半固体培养基中，该培养基补充有10%（体积/体积）来自鸡巨噬细胞系MQ-NCSU的条件培养基（CM）。条件培养基是通过将MQ-NCSU细胞在LM-HAHN（CMI）或RPMI-1640（CMII）生长培养基中培养获得的。对照培养物仅含有LM-HAHN或RPMI培养基。在CMI存在的情况下，骨髓细胞主要分化为粒细胞集落（实验1 = 84±9.2；实验2 = 105±5）。在对照培养物中未观察到集落。用脂多糖（LPS）刺激MQ-NCSU细胞产生的CM可使骨髓细胞主要分化为巨噬细胞集落（CMI中为122±16.3，CMII中为92±5.6）。这些数据表明，MQ-NCSU细胞自发分泌一种具有促进粒细胞分化潜能的因子。然而，在用LPS刺激后，分泌的因子具有巨噬细胞集落刺激潜能（M-CSF），与重组鸡骨髓单核细胞生长因子（r-cMGF）的活性相比，其活性相似。另一种来自鸡成纤维细胞的CM（FCM）在K系来亨鸡和艾维茵×艾维茵肉鸡的骨髓细胞上进行了测试。三个实验的数据表明，来自K系鸡的25×10³个骨髓细胞产生的巨噬细胞和粒细胞集落（82±14）比来自肉鸡的（56±12）更多。本研究表明，禽类细胞因子具有祖细胞分化潜能，且这种活性取决于细胞因子的来源及其靶细胞。
