Schuster V, Seidenspinner S, Kreth H W
Children's Hospital, University of Würzburg, Germany.
J Med Virol. 1996 Jan;48(1):114-20. doi: 10.1002/(SICI)1096-9071(199601)48:1<114::AID-JMV18>3.0.CO;2-G.
An EBV type 1 variant strain was detected in two Turkish siblings (boy and girl), who both suffered and died from similar progressive Epstein-Barr virus (EBV)-associated lymphoproliferative disease. Molecular characterisation of this EBV isolate revealed a 51bp-deletion and six nucleotide changes within the Epstein-Barr nuclear antigen 2 (EBNA2). Both isolates contained EBV type 2 sequences in the Epstein-Barr virus-encoded small RNAs (EBER), which are 40 kb proximal to EBNA2. Sequencing of the EBV isolates in a region of Epstein-Barr nuclear antigen 3 (EBNA3a), which is 40 kb distal to EBNA2, revealed the normal EBV type 1 sequence of laboratory strain B95-8. This EBV isolate may represent a distinct wild type EBV strain with altered biological properties. It is suggested that this EBNA2-variant strain may be responsible at least in part for the severe clinical course in both affected children.
在两名土耳其同胞（一男一女）中检测到1型爱泼斯坦-巴尔病毒（EBV）变异株，他们均患有相似的进行性EB病毒（EBV）相关淋巴增殖性疾病并死亡。对该EBV分离株的分子特征分析显示，在爱泼斯坦-巴尔核抗原2（EBNA2）内有一个51bp的缺失和六个核苷酸变化。两个分离株在EBV编码的小RNA（EBER）中均含有2型EBV序列，EBER位于EBNA2近端40 kb处。对位于EBNA2远端40 kb的爱泼斯坦-巴尔核抗原3（EBNA3a）区域的EBV分离株进行测序，结果显示为实验室菌株B95-8的正常1型EBV序列。这种EBV分离株可能代表一种具有改变生物学特性的独特野生型EBV菌株。提示这种EBNA2变异株可能至少部分导致了两名患病儿童的严重临床病程。
