Pham T N, Hayashi K, Takano R, Nakazawa H, Mori H, Ichida M, Itoh M, Eguchi M, Matsubara F, Hara S
Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institue of Technology.
J Biochem. 1996 Jun;119(6):1080-5. doi: 10.1093/oxfordjournals.jbchem.a021351.
Fungal protease inhibitor F (FPI-F) from silkworm hemolymph is a novel serine protease inhibitor of the Bombyx family. The cDNA of FPI-F was introduced into a baculovirus vector and a recombinant virus was isolated and plaque-purified. The protease inhibitory activities increased in the culture medium of insect cells and in the hemolymph of silkworms infected with the recombinant virus. Judged from the behavior on ion-exchange and reversed-phase chromatographies, amino acid compositions, amino-terminal sequences, and CD spectra, the recombinant FPI-F was identical with native FPI-F. Infection with the recombinant virus caused inhibition of larval development of the silkworm. However, the degree of the effect was different in two strains, Shinryukaku and Taiheichoan, indicating that the selection of the strain of silkworm is important in using the baculovirus expression system.
来自家蚕血淋巴的真菌蛋白酶抑制剂F（FPI-F）是家蚕家族一种新型的丝氨酸蛋白酶抑制剂。将FPI-F的cDNA导入杆状病毒载体，分离出重组病毒并进行空斑纯化。在昆虫细胞培养基以及感染重组病毒的家蚕血淋巴中，蛋白酶抑制活性均有所增加。从离子交换色谱、反相色谱行为、氨基酸组成、氨基末端序列以及圆二色谱判断，重组FPI-F与天然FPI-F相同。感染重组病毒会导致家蚕幼虫发育受到抑制。然而，在两个品系“新绿蠋”和“太和长庵”中，这种影响的程度有所不同，这表明在家蚕杆状病毒表达系统的应用中，家蚕品系的选择很重要。
