Krawczyñski vel Krawczyk T, Trojanowicz M, Lewenstam M, Moszczynska A
Department of Chemistry, University of Warsaw, Pasteura, Poland.
Biosens Bioelectron. 1996;11(11):1155-65. doi: 10.1016/0956-5663(96)82338-4.
In the lactate biosensor, electrodeposited poly(o-phenylenediamine) serves as a convenient matrix for the immobilization of lactate oxidase, but does not provide sufficient discrimination from several interfering species present in physiological fluids. Their effect, however, can be eliminated by additional modification of the working Pt electrode with a bilayer of electrodeposited polypyrrole/polyphenol. Despite continued decrease in biosensor sensitivity, the newly developed three-layer solid-state biosensor was successfully applied in flow-injection determination of lactate in both undiluted and diluted human blood serum samples over a 10 day period. For the lactate concentration range 0.2-5.0 mM in several series of measurements the correlation coefficient values for comparison with photometric determination using a DuPont dimension clinical analyzer were between 0.96 and 0.99. The reproducibility measured for 1:10 diluted serum was 0.6%. The detection limit was estimated as 2 microM.
在乳酸生物传感器中，电沉积的聚邻苯二胺作为固定乳酸氧化酶的便捷基质，但对于生理流体中存在的几种干扰物质无法提供足够的区分能力。然而，通过在工作铂电极上额外电沉积聚吡咯/多酚双层进行修饰，可以消除它们的影响。尽管生物传感器的灵敏度持续下降，但新开发的三层固态生物传感器在10天内成功应用于未稀释和稀释的人血清样品中乳酸的流动注射测定。在几个系列的测量中，对于0.2 - 5.0 mM的乳酸浓度范围，与使用杜邦Dimension临床分析仪进行光度测定相比，相关系数值在0.96至0.99之间。1:10稀释血清的测量重现性为0.6%。检测限估计为2 microM。
