基于多酚氧化酶包埋法制备用于测定苯酚的聚吡咯 - 聚乙烯磺酸盐复合膜生物传感器。
Preparation of a polypyrrole-polyvinylsulphonate composite film biosensor for determination of phenol based on entrapment of polyphenol oxidase.
作者信息
Arslan Halit, Arslan Fatma
机构信息
Department of Chemistry, Gazi University, Ankara, Turkey.
出版信息
Artif Cells Blood Substit Immobil Biotechnol. 2011 Oct;39(5):341-5. doi: 10.3109/10731199.2011.585616.
Abstract: In this paper, a novel amperometric phenol biosensor with immobilization of polyphenol oxidase (tyrosinase) on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique on the surface of a platinum electrode. The amperometric determination is based on the electrochemical reduction of quinon generated in the enzymatic reaction of phenol. The effects of pH and temperature were investigated and optimum parameters were found to be 8.0 and 30 °C, respectively. The linear working range of the electrode was 1.0 × 10(-7) - 5.0 × 10(-6) M. The storage stability and operation stability of the enzyme electrode were also studied.
摘要
本文通过包埋技术在铂电极表面制备了一种新型的安培型苯酚生物传感器，该传感器将多酚氧化酶（酪氨酸酶）固定在电化学聚合的聚吡咯-聚乙烯磺酸盐（PPy-PVS）膜上。安培测定法基于苯酚酶促反应中生成的醌的电化学还原。研究了pH值和温度的影响，发现最佳参数分别为8.0和30℃。该电极的线性工作范围为1.0×10⁻⁷ - 5.0×10⁻⁶ M。还研究了酶电极的储存稳定性和操作稳定性。
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