Laberge S, Wu L, Olivenstein R, Xu L J, Renzi P M, Martin J G
Meakins-Christie Laboratories, Royal Victoria Hospital, Montreal, Quebec, Canada.
J Allergy Clin Immunol. 1996 Sep;98(3):617-27. doi: 10.1016/s0091-6749(96)70096-9.
CD8+ (OX-8+) T cells may suppress airway inflammation and airway responsiveness after allergen challenge.
We studied the effects of depletion of OX-8+ T cells on allergen-induced lung eosinophilia and airway responsiveness in the Sprague-Dawley rat.
Sprague-Dawley rats were sensitized to ovalbumin and challenged by aerosol 14 days later. Test animals received either low-dose (2 mg, n = 9) or high-dose (3 mg, n = 7) OX-8 monoclonal antibody (mAb), whereas controls (n = 8) received BALB/c ascites fluid. A fourth group of animals (n = 10) was not sensitized to ovalbumin and also received ascites fluid. Twenty-four hours after ovalbumin challenge, responsiveness to methacholine was measured, and lung inflammation was assessed in the large airways and small airways and parenchyma.
Circulating and airway CD8+ T cells were decreased by OX-8 mAb administration with greatest changes in animals treated with high-dose OX-8 mAb compared with controls (blood: 1.0% +/- 3.6% vs 18.7% +/- 3.9%, p < 0.05); (large airways: 2.5% +/- 1.2% vs 13.8% +/- 1.2%, p < 0.05). Ovalbumin challenge resulted in increases in macrophages and neutrophils in the small airways and parenchyma of sensitized compared with unsensitized rats (p < 0.05). High-dose OX-8 mAb further increased total leukocytes, attributable to increases in neutrophils and eosinophils, retrieved from the large airways and small airways and parenchyma compared with other groups (p < 0.05). Airway responsiveness to methacholine was not significantly different between control and ovalbumin-challenged animals and was not augmented by OX-8 pretreatment.
CD8+ T cells modulate the extent of allergen-induced airway inflammation. However, the enhancement of inflammation was not sufficient to affect airway responsiveness.
CD8 +（OX - 8 +）T细胞可能在变应原激发后抑制气道炎症和气道反应性。
我们研究了去除OX - 8 + T细胞对Sprague-Dawley大鼠变应原诱导的肺嗜酸性粒细胞增多和气道反应性的影响。
将Sprague-Dawley大鼠用卵清蛋白致敏，14天后进行雾化激发。试验动物接受低剂量（2mg，n = 9）或高剂量（3mg，n = 7）的OX - 8单克隆抗体（mAb），而对照组（n = 8）接受BALB/c腹水。第四组动物（n = 10）未用卵清蛋白致敏，也接受腹水。卵清蛋白激发24小时后，测量对乙酰甲胆碱的反应性，并评估大气道、小气道和实质中的肺部炎症。
与对照组相比，给予OX - 8 mAb后循环和气道CD8 + T细胞减少，高剂量OX - 8 mAb处理的动物变化最大（血液：1.0%±3.6%对18.7%±3.9%，p < 0.05）；（大气道：2.5%±1.2%对13.8%±1.2%，p < 0.05）。与未致敏大鼠相比，卵清蛋白激发导致致敏大鼠小气道和实质中的巨噬细胞和中性粒细胞增加（p < 0.05）。与其他组相比，高剂量OX - 8 mAb进一步增加了从大气道、小气道和实质中回收的总白细胞，这归因于中性粒细胞和嗜酸性粒细胞的增加（p < 0.05）。对照组和卵清蛋白激发的动物之间对乙酰甲胆碱的气道反应性无显著差异，并且OX - 8预处理未增强该反应性。
CD8 + T细胞调节变应原诱导的气道炎症程度。然而，炎症的增强不足以影响气道反应性。
